Carnosine
L-Carnosine
Key Specifications
| CAS Number | 305-84-0 |
| Purity | ≥98.0% |
| Molecular Formula | C₉H₁₄N₄O₃ |
| Molecular Weight | 226.23 g/mol |
| Appearance | White crystalline powder |
| Solubility | Freely soluble in water |
| Storage | Room temperature, dry |
| Shelf Life | 36 months |
CAS 305-84-0
What Is Carnosine?
L-Carnosine is a naturally occurring dipeptide formed from the amino acids beta-alanine and L-histidine (CAS 305-84-0; molecular formula C9H14N4O3; MW 226.23 g/mol). It occurs endogenously at high concentrations in the skeletal muscle, cardiac tissue, and brain of vertebrates, where it contributes to intracellular pH buffering and cellular protection. For commercial supply, bulk L-carnosine is produced through a biotechnology-based route: the two precursor amino acids, L-histidine and beta-alanine, are first obtained by microbial fermentation, then joined through a controlled enzymatic coupling step that forms the characteristic beta-amino peptide bond. The resulting dipeptide is purified by crystallization and dried into a white crystalline powder of ≥98.0% purity, suitable for dietary supplement, cosmetic, and pharmaceutical-grade raw material applications.
Other Names
Key Features
High, Verified Purity
Each production batch is assayed at ≥98.0% purity by HPLC, with a batch-specific certificate of analysis issued for every shipment.
Controlled Bio-Based Production
Precursor amino acids are produced via microbial fermentation and joined through a tightly controlled enzymatic coupling process, reducing residual solvents and by-products versus purely chemical routes.
Consistent Crystalline Form
Supplied as a free-flowing white crystalline powder with consistent particle size, appearance, and odor from batch to batch.
Full Contaminant Screening
Every lot is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits to meet food-grade, cosmetic-grade, and pharmaceutical raw material requirements.
Flexible Bulk & OEM Supply
Available in bulk quantities with customizable packaging, particle size, and private-label documentation to support OEM/ODM programs.
| Molecular Formula | C₉H₁₄N₄O₃ |
| Molecular Weight | 226.23 g/mol |
| Appearance | White crystalline powder |
| Solubility | Freely soluble in water |
| Storage Condition | Room temperature, dry |
| Shelf Life | 36 months |
| Grade 1 | Supplement Grade · ≥98.0% · COA |
End-Product Applications
Mechanism & Benefits
Antioxidant and anti-glycation dipeptide. Protects muscle cells, improves athletic performance and delays aging.
Packaging Options
1 kg 5 kg 25 kg
Lead Time
5–7 business days
Minimum Order
1 kg
Custom Packaging
Custom packaging (foil bags, HDPE drums), GMP labeling, and export documentation are available. Contact us for specifics.Certifications
ISO
ISO
ISO9001
ISO 9001
Manufacturing Process
Precursor Amino Acid Fermentation
Selected microbial strains ferment renewable feedstocks to produce the two building-block amino acids, L-histidine and beta-alanine, in separate fermentation streams.
Precursor Recovery and Purification
Fermentation broths are clarified, and each amino acid is recovered by ion-exchange chromatography and crystallization to high purity.
Enzymatic Dipeptide Coupling
Purified L-histidine and beta-alanine are combined under controlled temperature and pH in a biocatalytic coupling reaction that forms the beta-amino peptide bond characteristic of carnosine.
Reaction Quench and Clarification
The coupling reaction is quenched, and the crude solution is filtered to remove residual biomass, spent catalyst, and insoluble by-products.
Chromatographic Purification
The clarified solution undergoes ion-exchange and/or resin-based chromatography to remove unreacted precursors and trace impurities.
Crystallization
The purified carnosine solution is concentrated and crystallized under controlled cooling conditions to yield uniform crystals.
Drying and Milling
Crystals are dried under vacuum, then milled and sieved to achieve the target particle size and a free-flowing white powder.
Quality Control and Packaging
Each batch is tested against the full quality specification (assay, heavy metals, microbial limits) before being packaged under controlled conditions for shipment.
Quality Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White crystalline powder | Conforms |
| Odor | Characteristic, no off-odor | Conforms |
| Assay (HPLC) | ≥98.0% | 99.1% |
| pH (1% aqueous solution) | 7.0 - 8.5 | 7.8 |
| Loss on Drying | ≤0.5% | 0.22% |
| Particle Size / Crystal Form | Fine crystalline powder, through 80 mesh ≥95% | Conforms |
| Lead (Pb) | ≤2 mg/kg | <1 mg/kg |
| Arsenic (As) | ≤2 mg/kg | <1 mg/kg |
| Mercury (Hg) | ≤1 mg/kg | <0.5 mg/kg |
| Cadmium (Cd) | ≤1 mg/kg | <0.5 mg/kg |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25 g | Negative |
| Salmonella | Negative/25 g | Negative |
Purity & Assay Method
Purity is determined by high-performance liquid chromatography (HPLC), typically using a reverse-phase or ion-pair column referenced against a certified L-carnosine standard, with content calculated as the area percentage of the main peak. Buyers should confirm the specific HPLC method, mobile phase, and reference standard applied, and request a batch-specific certificate of analysis (COA) confirming the material meets the ≥98.0% purity specification before finalizing an order.
Solubility & Formulation
L-carnosine is freely soluble in water, making it well suited for aqueous formulations such as functional beverages, effervescent tablets, oral solutions, and water-based cosmetic serums. It is comparatively less soluble in ethanol and other organic solvents, so formulators working with anhydrous or oil-based systems may need co-solvents, micronized grades, or microencapsulation technology to improve dispersibility. As a zwitterionic dipeptide, its solubility and stability can be influenced by formulation pH and the presence of reducing sugars (Maillard-type interactions), which should be evaluated during product development.
Available Product Forms
Bulk Carnosine Powder
High-purity raw powder for supplement factories, research organizations, ingredient distributors, and contract manufacturers.
Carnosine Capsules
Custom capsule strength, shell material, bottle count, excipients, and label design for private-label brands.
Carnosine Tablets
Tablet formulation, granulation, compression, coating, bottle packaging, and custom labeling support.
Carnosine Powder Blends
Carnosine can be blended with complementary actives (antioxidants, other longevity ingredients, vitamins, minerals) per customer formula.
Can you confirm the exact purity and provide a certificate of analysis?+
Yes. Every batch is assayed at ≥98.0% purity by HPLC, and a batch-specific certificate of analysis (COA) covering assay, heavy metals, and microbial limits is issued with each shipment.
How is your L-carnosine manufactured — is it fermentation-based or chemically synthesized?+
Our L-carnosine is produced through a biotechnology-based process: the precursor amino acids are obtained via microbial fermentation, then joined by a controlled enzymatic coupling step. This is not derived from animal tissue extraction.
Is L-carnosine soluble in water, and can it be used in beverage formulations?+
Yes, L-carnosine is freely soluble in water, which makes it suitable for beverages, effervescent products, and other aqueous formulations. Solubility in ethanol and oils is limited, so oil-based systems may require additional formulation support.
Can we request a sample before placing a bulk order?+
Yes, small samples are available on request for formulation trials and quality evaluation prior to committing to a bulk order.
Do you support third-party testing or independent lab verification?+
Yes. We operate under ISO/ISO9001-certified quality management and are glad to accommodate third-party or customer-designated laboratory testing of samples prior to or after shipment.
Can you provide OEM/private-label packaging and documentation?+
Yes, we support OEM/ODM programs including custom labeling, private-label documentation, and packaging tailored to customer specifications.
What packaging options are available for bulk shipment?+
Standard bulk packaging is in moisture-proof, double-lined drums or bags (e.g., 1 kg, 5 kg, and 25 kg), with custom sizes and packaging materials available on request.
What is the MOQ, and how can we get a quotation?+
MOQ is flexible and depends on whether the order is for a trial sample or a bulk purchase; please contact us with your target quantity and application to receive a formal quotation along with the current COA and specification sheet.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Batch-specific COA & full traceability on every shipment
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party laboratory testing
Documents Available
- ·Certificate of Analysis (COA)
- ·Product specification sheet
- ·Material Safety Data Sheet (MSDS)
- ·Manufacturing flow chart
- ·HPLC test report
- ·Heavy-metal test report
- ·Microbiological test report
- ·Non-GMO / allergen statement
- ·Country-of-origin documentation
- ·Packing list & commercial invoice
Certificate availability should be confirmed with our sales team before ordering.
Sample Certificate of Analysis
PathGene Biotech Co., Ltd.
南京派琪生物科技有限公司
CERTIFICATE OF ANALYSIS
Layout example — not an issued batch record
Product Name
Carnosine
CAS No.
305-84-0
Batch No.
PG-SAMPLE-000000
Packaging
Issued upon order confirmation
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White crystalline powder | Conforms |
| Odor | Characteristic, no off-odor | Conforms |
| Assay (HPLC) | ≥98.0% | 99.1% |
| pH (1% aqueous solution) | 7.0 - 8.5 | 7.8 |
| Loss on Drying | ≤0.5% | 0.22% |
| Particle Size / Crystal Form | Fine crystalline powder, through 80 mesh ≥95% | Conforms |
| Lead (Pb) | ≤2 mg/kg | <1 mg/kg |
| Arsenic (As) | ≤2 mg/kg | <1 mg/kg |
| Mercury (Hg) | ≤1 mg/kg | <0.5 mg/kg |
| Cadmium (Cd) | ≤1 mg/kg | <0.5 mg/kg |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25 g | Negative |
| Salmonella | Negative/25 g | Negative |
Conclusion: Conforms to internal quality specifications
The COA issued with your order will carry the actual batch number, manufacture/test dates, and QC signature.
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Interested in Carnosine?
Our R&D team will respond to your inquiry or sample request within 24 hours.
