NK
CAS 133876-92-3
Enzyme Activity≥20,000 FU/g
Enzyme preparation for functional-nutrition formulation screening
Nattokinase powder (CAS 133876-92-3) is supplied by enzyme activity: the standard grade is ≥20,000 FU/g, with custom ≥5,000 and ≥40,000 FU/g grades also listed. FU/g describes activity, not a purity percentage. Batch COA and evaluation samples are available; MOQ 1 kg, with 5 kg/carton and 25 kg/drum pack options. nutrition & functional ingredients
Quote / sample & delivery
Key Specifications
| Product Positioning | Enzyme preparation for functional-nutrition formulation screening |
| Application Scenarios | enzyme activity assays · functional-nutrition capsule development · process-stability research |
| CAS Number | 133876-92-3 |
| Enzyme Activity | ≥20,000 FU/g |
| Molecular Formula | Enzyme |
| Molecular Weight | ~27,728 Da |
| Appearance | Off-white to light yellow powder |
| Solubility | Not specified in the referenced COA; verify in the target formulation |
| Storage | Sealed packaging; store cool, dry and away from light |
| Shelf Life | 24 months |
Procurement & delivery
Supply details confirmed by intended use, volume and target market
Nattokinase (NK, CAS 133876-92-3) is a fibrinolytic serine protease of the subtilisin family (EC 3.4.21.62) that occurs naturally during the fermentation of soybeans by Bacillus subtilis var. natto, the bacterium used to make the traditional Japanese food natto. As a protein enzyme it has no fixed small-molecule formula; its polypeptide chain comprises roughly 275 amino acid residues with an approximate molecular weight of 27,728 Da. Commercial bulk nattokinase is manufactured through controlled submerged or solid-state fermentation of selected Bacillus subtilis natto strains in soy-based nutrient media, followed by cell separation, ultrafiltration, chromatographic purification, activity standardization, and spray-drying into a stable powder.
Standard 20,000 FU/g Enzyme Activity
The standard activity specification is ≥20,000 FU/g. Official COA batch PGNK-25120301 recorded 23,352 FU/g; supplied material should always be confirmed against its own batch COA.
5,000 & 40,000 FU/g Custom Grades
Official COAs confirm custom specifications of ≥5,000 FU/g (batch result 5,228 FU/g) and ≥40,000 FU/g (batch result 43,512 FU/g). Confirm available activity grade, packaging and lead time before ordering.
COA-Controlled Powder Quality
The 20,000 FU/g COA specifies light yellow to brown powder with brown spots, characteristic smell and no visible foreign impurities. Water is specified at ≤5.0%; the referenced batch result was 4.8%.
Microbiological & Aflatoxin B1 Controls
The official COA includes total microbial count, mold, coliform and aflatoxin B1 release limits. Final suitability for the intended finished product and market remains the buyer's responsibility.
Flexible Bulk & OEM/Private-Label Supply
Available in bulk drum or bag packaging with customizable activity grades, particle size, and private-label documentation to fit formulator requirements.
Test items, specifications and results for the listed batch are shown here. View or request a COA in the quality documents section.
Batch No.
PGNK-260625
Test date
Specification
In-house
Pack size
1kg.10kg/bag
Storage
Sealed packaging; store cool, dry and away from light
Conclusion
Conforms
| Test Item | Specification | Result |
|---|---|---|
| Appearance | Light yellow to brown with brown spots | Complies |
| Smell | Unique smell and taste | Conforms |
| State | Powder | Complies |
| Impurity | Free from visible foreign impurities | Complies |
| Nattokinase Activity | ≥20,000 FU/g | 23,002 FU/g (PGNK-260625) |
| Water | ≤5.0% | 4.8% |
| Total Microbial Count | n=5; c=2; m=3×10⁴; M=10⁵ | <10, <10, <10, <10, <10 |
| Mold | ≤25 CFU/g | <10 CFU/g |
| Coliform | n=5; c=1; m=20; M=30 | <10, <10, <10, <10, <10 |
| Aflatoxin B1 | ≤5.0 μg/kg | Complies |
View an available COA in the quality documents section. Check the document for its product form and batch; request a signed COA for your purchasing or shipment batch separately.
Hard capsules
Powders
Softgels
Tablets
Sublingual tablets
Oral dissolving films
Use FU/g activity for loading, not protein content or HPLC purity.
Complies
Impurity
23,002 FU/g (PGNK-260625)
Nattokinase Activity
4.8%
Water
PGNK-260625 · Results apply to this lot; interpret purity, assay and water on their respective bases.
Composition candidates, processing sequences and tests support pilot design. Confirm candidate excipients and processes using this material in the actual finished formulation.
Application examples follow the materials, formats and findings actually studied. Combination studies, single-ingredient studies, commercial precedents and development candidates are distinguished.
NK is activity-standardized while red yeast rice has separate active constituents; a capsule trial illustrates independent specification within one format.
Show research and formulation insights +Hide details −Combination / selection rationale: NK is activity-standardized while red yeast rice has separate active constituents; a capsule trial illustrates independent specification within one format.
Published evidence / source details: A 2024 trial analysed 178 participants over 90 days across blend, single-ingredient and placebo groups. Each blend capsule measured 610.3 FU NK and 1.5 mg monacolins; the combination group showed improvements in triglycerides, total cholesterol and diastolic pressure.
Development insights: Calculate NK loading from lot FU/g and red-yeast-rice actives separately. Recheck activity after processing; control monacolins, citrinin and market requirements, and compare activity retention with each partner.
Evidence scope: Evidence concerns a specified population and formula, not a treatment claim or general intake recommendation.
2024 · NK/RYR randomized study · DOI 10.3389/fnut.2024.1380727 ↗Equal powder mass does not imply equal activity; compare grades using activity specification and actual lot values.
Show research and formulation insights +Hide details −Combination / selection rationale: Equal powder mass does not imply equal activity; compare grades using activity specification and actual lot values.
Published evidence / source details: The capsule trial reports FU per capsule separately from red-yeast-rice actives, illustrating why enzyme activity and ingredient mass must be distinguished.
Development insights: For a 2000-FU loading example, nominal 20,000/5000/40,000-FU/g powders require 100/400/50 mg, respectively. Adjust to lot activity and measured process loss; recheck blend and end-of-storage activity.
Evidence scope: 2000 FU is a loading example, not intake advice or a finished-product guarantee.
2024 · Related capsule-format reference · NK/RYR trial ↗Literature-Supported Research Directions
Nattokinase is a proteolytic enzyme of interest for fibrinolysis, blood-pressure and vascular-risk-marker research. Some short-term randomized trials reported blood-pressure changes, while a longer atherosclerosis trial did not improve its primary endpoints; claims should remain cautious.
Citation: Kim et al., Hypertension Research (2008), eight-week randomized trial of nattokinase and blood pressure.View source
Nattokinase can be used in development work on coagulation/fibrinolysis, hemorheology and cardiovascular-risk markers. Long-term randomized evidence indicates that short-term biomarker changes should not be presented as proven prevention of atherosclerosis or cardiovascular events.
Citation: Hodis et al., American Heart Journal (2021), three-year randomized trial showing no improvement in subclinical atherosclerosis progression.View source
Note: For ingredient R&D and scientific information; findings depend on model, dose, formulation and population and do not constitute a treatment claim or efficacy guarantee.
Lead Time
3–5business days
Standard products are generally in stock; confirm the exact lead time with us.
Minimum Order
1 kg
Samples, pilot trials, and commercial volumes are supported.
Foil Pouch
Sealed, moisture-protective inner packaging
Fiber Drum
Standard outer packaging for bulk transport
Bulk Shipping
Palletized and stretch-wrapped for protection
Ambient storage: foil pouch + fiber drum; temperature controlled: foil pouch + insulated box/carton. Confirm by product.
Custom packaging: Custom foil bags or HDPE drums, GMP label printing, and export documentation are available. Contact us for a quote.Images are packaging illustrations.
Certifications
Strain Selection & Seed Culture Preparation
A high-yield Bacillus subtilis var. natto production strain is activated and expanded through a multi-stage seed culture to reach the inoculum density required for fermentation.
Fermentation Medium Preparation
A soy-based nutrient medium (soybean/soy protein, glucose or glycerol, nitrogen sources, and trace minerals) is formulated and sterilized to support enzyme-producing growth.
Submerged or Solid-State Fermentation
The seed culture is inoculated into bioreactors (or solid-state trays) under controlled temperature, pH, agitation, and aeration for a fixed fermentation cycle to maximize nattokinase secretion.
Cell Separation & Clarification
Fermentation broth is clarified by centrifugation and/or filtration to remove bacterial biomass and coarse solids, yielding a clear crude enzyme solution.
Ultrafiltration & Concentration
The crude enzyme solution is concentrated via ultrafiltration membranes, which also removes low-molecular-weight impurities and reduces downstream processing volume.
Chromatographic Purification
Ion-exchange and/or gel filtration chromatography is used to separate the enzyme fraction from residual proteins, pigments, and fermentation by-products, helping support activity-grade standardization.
Activity Standardization & Spray Drying
The purified enzyme solution is assayed for fibrinolytic activity, adjusted/standardized with approved carriers as needed, and spray-dried into a stable powder.
Quality Control & Packaging
Finished powder is released against the agreed enzyme-activity grade and the applicable COA tests, then packaged under moisture-controlled conditions for storage and shipment.
Nattokinase is specified and supplied by enzyme activity, expressed as FU/g, rather than by a purity percentage. The standard grade is ≥20,000 FU/g; custom ≥5,000 and ≥40,000 FU/g grades are supported. Review the COA for the supplied lot because activity is a batch-specific release result.
The referenced COAs do not state solubility or a finished-formulation recommendation. Confirm dispersion, activity retention, processing compatibility and stability in the target formulation before scale-up.
No. FU/g reports fibrinolytic activity per gram of the supplied powder. It is not a mass percentage of nattokinase. Compare grades using the same activity method and reporting basis, then review composition and carrier information separately. The standard grade is ≥20,000 FU/g; its delivered lot has its own measured activity result.
The standard grade is ≥20,000 FU/g, with custom ≥5,000 and ≥40,000 FU/g grades available. Each supplied batch is accompanied by its own COA showing the nattokinase activity result in FU/g; the referenced 20,000 FU/g batch PGNK-25120301 recorded 23,352 FU/g.
It is produced by controlled fermentation of a selected Bacillus subtilis var. natto strain on a soy-based nutrient medium, followed by separation, ultrafiltration, chromatographic purification, and spray-drying — the same biological pathway by which nattokinase naturally forms during natto fermentation.
Nattokinase formulation development should focus on enzyme-activity retention. Test dispersion, processing temperature, pH, excipient compatibility and storage stability in the target system. Raw-material activity is reported in FU/g; assess the finished format using its inclusion level and measured retained activity.
Yes, samples are available for evaluation; please contact our sales team to confirm current sample size, cost policy, and lead time.
Yes, buyers are welcome to send samples to an independent laboratory of their choice for verification of enzyme activity, water, microbiological limits and other agreed specifications.
Yes, we support OEM and private-label supply, including customizable activity grades, particle size, packaging, and labeling documentation to match your formulation and branding needs.
Nattokinase powder pack options are 5 kg/carton, 25 kg/drum, Custom packaging. Special inner-packaging, labeling and transport requirements are agreed separately for the order.
The raw-material MOQ for nattokinase powder is 1 kg, with pack options of 5 kg/carton, 25 kg/drum, Custom packaging. The standard activity grade is ≥20,000 FU/g; custom 5,000 or 40,000 FU/g grades are agreed separately.
Required ingredient mass (g) equals target activity per unit (FU) divided by ingredient activity (FU/g). At nominal 20,000 FU/g, a 2000-FU loading example requires 0.1 g; use the lot activity and measured processing loss in development. Retest finished-product activity during storage. This is a loading calculation, not an intake recommendation.
Documents are subject to product and batch availability; third-party reports are provided only when applicable.
Quality documents
The specification sheet defines acceptance limits. The COA shows actual batch results, which may vary by batch. Third-party reports are provided only for products or batches that have been independently tested.
Stable acceptance criteria · Available on request
Actual test data · Check the COA for the applicable batch
Technical Resources
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