NK
Nattokinase
Key Specifications
| CAS Number | 133876-92-3 |
| Purity | ≥98.0% (protein basis) |
| Molecular Formula | Enzyme |
| Molecular Weight | ~27,728 Da |
| Appearance | Off-white to light yellow powder |
| Solubility | Soluble in water |
| Storage | 2–8°C, protect from moisture |
| Shelf Life | 24 months |
CAS 133876-92-3
What Is NK?
Nattokinase (NK, CAS 133876-92-3) is a fibrinolytic serine protease of the subtilisin family (EC 3.4.21.62) that occurs naturally during the fermentation of soybeans by Bacillus subtilis var. natto, the bacterium used to make the traditional Japanese food natto. As a protein enzyme it has no fixed small-molecule formula; its polypeptide chain comprises roughly 275 amino acid residues with an approximate molecular weight of 27,728 Da. Commercial bulk nattokinase is manufactured through controlled submerged or solid-state fermentation of selected Bacillus subtilis natto strains in soy-based nutrient media, followed by cell separation, ultrafiltration, chromatographic purification, activity standardization, and spray-drying into a stable powder.
Other Names
Key Features
High Purity, Standardized Activity
Protein purity of ≥98.0% with fibrinolytic activity standardized in fibrinolytic units (FU/g), ensuring consistent potency batch to batch.
Controlled Fermentation from Selected B. subtilis natto Strains
Produced under tightly controlled fermentation parameters (temperature, pH, aeration, feed) using screened high-yield Bacillus subtilis var. natto strains for reproducible quality.
Consistent Off-White Powder Form
Supplied as an off-white to light yellow, free-flowing powder that is soluble in water, convenient for downstream weighing, blending, and encapsulation.
Rigorous Contaminant Testing
Every batch is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast & mold, E. coli, Salmonella) before release.
Flexible Bulk & OEM/Private-Label Supply
Available in bulk drum or bag packaging with customizable activity grades, particle size, and private-label documentation to fit formulator requirements.
| Molecular Formula | Enzyme |
| Molecular Weight | ~27,728 Da |
| Appearance | Off-white to light yellow powder |
| Solubility | Soluble in water |
| Storage Condition | 2–8°C, protect from moisture |
| Shelf Life | 24 months |
| Grade 1 | Food Grade · ≥20000 FU/g · COA |
End-Product Applications
Mechanism & Benefits
Fibrinolytic enzyme from B. subtilis natto fermentation. Dissolves blood clots, improves circulation.
Packaging Options
1 kg 5 kg 25 kg
Lead Time
7–10 business days
Minimum Order
1 kg
Custom Packaging
Custom packaging (foil bags, HDPE drums), GMP labeling, and export documentation are available. Contact us for specifics.Certifications
ISO
ISO
ISO9001
ISO 9001
Manufacturing Process
Strain Selection & Seed Culture Preparation
A high-yield Bacillus subtilis var. natto production strain is activated and expanded through a multi-stage seed culture to reach the inoculum density required for fermentation.
Fermentation Medium Preparation
A soy-based nutrient medium (soybean/soy protein, glucose or glycerol, nitrogen sources, and trace minerals) is formulated and sterilized to support enzyme-producing growth.
Submerged or Solid-State Fermentation
The seed culture is inoculated into bioreactors (or solid-state trays) under controlled temperature, pH, agitation, and aeration for a fixed fermentation cycle to maximize nattokinase secretion.
Cell Separation & Clarification
Fermentation broth is clarified by centrifugation and/or filtration to remove bacterial biomass and coarse solids, yielding a clear crude enzyme solution.
Ultrafiltration & Concentration
The crude enzyme solution is concentrated via ultrafiltration membranes, which also removes low-molecular-weight impurities and reduces downstream processing volume.
Chromatographic Purification
Ion-exchange and/or gel filtration chromatography is used to separate nattokinase from residual proteins, pigments, and fermentation by-products, raising protein purity to ≥98%.
Activity Standardization & Spray Drying
The purified enzyme solution is assayed for fibrinolytic activity, adjusted/standardized with approved carriers as needed, and spray-dried into a stable powder.
Quality Control & Packaging
Finished powder is tested for purity, activity, heavy metals, and microbial limits, then packaged under moisture-controlled conditions for storage and shipment.
Quality Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | Off-white to light yellow powder | Off-white powder, conforms |
| Odor | Characteristic, no off-odor | Conforms |
| Protein Purity (HPLC/Kjeldahl) | ≥98.0% | 98.3% |
| Fibrinolytic Activity (fibrin plate method) | ≥20,000 FU/g | 20,600 FU/g |
| Loss on Drying | ≤5.0% | 3.6% |
| Particle Size (through 80 mesh) | ≥95% | 98% |
| pH (1% aqueous solution) | 6.0-7.5 | 6.7 |
| Lead (Pb) | ≤2 mg/kg | <0.5 mg/kg |
| Arsenic (As) | ≤1 mg/kg | <0.5 mg/kg |
| Mercury (Hg) | ≤0.1 mg/kg | <0.05 mg/kg |
| Cadmium (Cd) | ≤1 mg/kg | <0.3 mg/kg |
| Total Plate Count | ≤1,000 CFU/g | <500 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <50 CFU/g |
| E. coli | Negative/25g | Negative |
| Salmonella | Negative/25g | Negative |
Purity & Assay Method
Protein purity is typically confirmed by HPLC and/or Kjeldahl nitrogen determination, while fibrinolytic potency is measured separately via a fibrin-plate or clot-lysis activity assay expressed in fibrinolytic units (FU/g) — purity and activity are not interchangeable specifications. Buyers should request the full Certificate of Analysis, confirm which activity assay method and reference standard were used, and where possible arrange independent third-party verification before large-volume procurement.
Solubility & Formulation
Nattokinase is soluble in water, typically forming a clear to slightly hazy solution suitable for aqueous processing. Because it is a protein enzyme, activity can be sensitive to heat, extreme pH, and prolonged exposure to moisture or oxygen, so formulators commonly use enteric-coated capsules or tablets to help protect the enzyme through the acidic stomach environment before release in the intestine, and recommend cool, dry storage plus moisture-barrier packaging to preserve potency through the product's shelf life.
Available Product Forms
Bulk NK Powder
High-purity raw powder for supplement factories, research organizations, ingredient distributors, and contract manufacturers.
NK Capsules
Custom capsule strength, shell material, bottle count, excipients, and label design for private-label brands.
NK Tablets
Tablet formulation, granulation, compression, coating, bottle packaging, and custom labeling support.
NK Powder Blends
NK can be blended with complementary actives (antioxidants, other longevity ingredients, vitamins, minerals) per customer formula.
How can I confirm the actual purity and activity of each batch?+
Each shipment is accompanied by a Certificate of Analysis showing protein purity (HPLC/Kjeldahl) and fibrinolytic activity (FU/g). We can also provide retained samples for buyer-side or third-party re-testing.
How is your nattokinase produced, and what is the raw material source?+
It is produced by controlled fermentation of a selected Bacillus subtilis var. natto strain on a soy-based nutrient medium, followed by separation, ultrafiltration, chromatographic purification, and spray-drying — the same biological pathway by which nattokinase naturally forms during natto fermentation.
Is nattokinase soluble in water, and how should it be formulated?+
Yes, it is soluble in water. For oral applications it is commonly formulated into enteric-coated capsules or tablets to help protect enzyme activity through the stomach; cool, dry, moisture-protected storage is recommended.
Can we request a free or paid sample before placing a bulk order?+
Yes, samples are available for evaluation; please contact our sales team to confirm current sample size, cost policy, and lead time.
Do you support independent third-party testing?+
Yes, buyers are welcome to send samples to an independent laboratory of their choice for verification of purity, activity, heavy metals, and microbial limits.
Do you offer OEM or private-label services?+
Yes, we support OEM and private-label supply, including customizable activity grades, particle size, packaging, and labeling documentation to match your formulation and branding needs.
What packaging options are available?+
Standard packaging includes moisture-proof aluminum foil bags or fiber drums (e.g., 1 kg, 5 kg, 25 kg), with custom packaging sizes available on request.
What is the MOQ, and how can I get a formal quotation?+
MOQ varies by activity grade and packaging format; please contact our sales team with your target specification and order volume for a formal quotation and lead time.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Batch-specific COA & full traceability on every shipment
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party laboratory testing
Documents Available
- ·Certificate of Analysis (COA)
- ·Product specification sheet
- ·Material Safety Data Sheet (MSDS)
- ·Manufacturing flow chart
- ·HPLC test report
- ·Heavy-metal test report
- ·Microbiological test report
- ·Non-GMO / allergen statement
- ·Country-of-origin documentation
- ·Packing list & commercial invoice
Certificate availability should be confirmed with our sales team before ordering.
Sample Certificate of Analysis
PathGene Biotech Co., Ltd.
南京派琪生物科技有限公司
CERTIFICATE OF ANALYSIS
Layout example — not an issued batch record
Product Name
NK
CAS No.
133876-92-3
Batch No.
PG-SAMPLE-000000
Packaging
Issued upon order confirmation
| Test Item | Specification | Result |
|---|---|---|
| Appearance | Off-white to light yellow powder | Off-white powder, conforms |
| Odor | Characteristic, no off-odor | Conforms |
| Protein Purity (HPLC/Kjeldahl) | ≥98.0% | 98.3% |
| Fibrinolytic Activity (fibrin plate method) | ≥20,000 FU/g | 20,600 FU/g |
| Loss on Drying | ≤5.0% | 3.6% |
| Particle Size (through 80 mesh) | ≥95% | 98% |
| pH (1% aqueous solution) | 6.0-7.5 | 6.7 |
| Lead (Pb) | ≤2 mg/kg | <0.5 mg/kg |
| Arsenic (As) | ≤1 mg/kg | <0.5 mg/kg |
| Mercury (Hg) | ≤0.1 mg/kg | <0.05 mg/kg |
| Cadmium (Cd) | ≤1 mg/kg | <0.3 mg/kg |
| Total Plate Count | ≤1,000 CFU/g | <500 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <50 CFU/g |
| E. coli | Negative/25g | Negative |
| Salmonella | Negative/25g | Negative |
Conclusion: Conforms to internal quality specifications
The COA issued with your order will carry the actual batch number, manufacture/test dates, and QC signature.
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Interested in NK?
Our R&D team will respond to your inquiry or sample request within 24 hours.
