PLP
Pyridoxal 5'-Phosphate
Key Specifications
| CAS Number | 54-47-7 |
| Purity | ≥98.0% |
| Molecular Formula | C₈H₁₀NO₆P |
| Molecular Weight | 247.14 g/mol |
| Appearance | Light yellow to yellow crystalline powder |
| Solubility | Soluble in water |
| Storage | 2–8°C, protect from light |
| Shelf Life | 24 months |
CAS 54-47-7
What Is PLP?
Pyridoxal 5'-phosphate (PLP, CAS 54-47-7) is the biologically active, phosphorylated coenzyme form of vitamin B6, chemically classified as a pyridoxal derivative with molecular formula C8H10NO6P and molecular weight 247.14 g/mol. In the body, PLP is generated endogenously from vitamin B6 precursors (pyridoxine, pyridoxal, pyridoxamine) via kinase and oxidase enzymes in the liver. For commercial bulk supply, PLP is manufactured starting from pyridoxine through a controlled phosphorylation and oxidation sequence — using either conventional chemical phosphorylating reagents or increasingly enzymatic/biocatalytic cascades built on pyridoxal kinase and pyridoxine-5'-phosphate oxidase — followed by purification and crystallization to yield a light yellow to yellow crystalline powder suitable for pharmaceutical and dietary supplement use.
Other Names
Key Features
Pharmaceutical-Grade Purity
Assayed at ≥98.0% by HPLC and manufactured to meet USP monograph expectations, ensuring consistent potency batch to batch for pharmaceutical and nutraceutical formulators.
Controlled Biocatalytic Production
Produced from pyridoxine through a tightly controlled phosphorylation-oxidation process, leveraging our enzymatic and synthetic-biology production platform to improve reaction selectivity and reduce process impurities.
Consistent Crystalline Appearance
Supplied as a uniform light yellow to yellow crystalline powder with controlled particle size, supporting reliable weighing, blending, and downstream processing.
Rigorous Contaminant Screening
Every batch is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella), with full documentation available on request.
Flexible Bulk & OEM Supply
Available in flexible bulk quantities with customizable packaging, labeling, and OEM/private-label options to support supplement, pharmaceutical, and premix manufacturers of varying scale.
| Molecular Formula | C₈H₁₀NO₆P |
| Molecular Weight | 247.14 g/mol |
| Appearance | Light yellow to yellow crystalline powder |
| Solubility | Soluble in water |
| Storage Condition | 2–8°C, protect from light |
| Shelf Life | 24 months |
| Grade 1 | USP Grade · ≥98.0% · USP |
End-Product Applications
Mechanism & Benefits
Active vitamin B6 form. Involved in protein metabolism and neurotransmitter synthesis, mood improvement.
Packaging Options
1 kg 5 kg 25 kg
Lead Time
5–7 business days
Minimum Order
1 kg
Custom Packaging
Custom packaging (foil bags, HDPE drums), GMP labeling, and export documentation are available. Contact us for specifics.Certifications
USP
US Pharmacopoeia
ISO
ISO
Manufacturing Process
Precursor Sourcing
High-purity pyridoxine (vitamin B6) is sourced and qualified as the starting precursor, with incoming material verified against internal specifications before entering the production line.
Biocatalyst/Reagent Preparation
Pyridoxal kinase and pyridoxine-5'-phosphate oxidase biocatalysts (or, in the conventional route, chemical phosphorylating reagents) are prepared and activity-verified prior to use.
Phosphorylation
Pyridoxine is enzymatically (or chemically) phosphorylated at the 5'-hydroxymethyl position to generate pyridoxine-5'-phosphate, with ATP or an equivalent phosphoryl donor supplied and regenerated in situ for the biocatalytic route.
Oxidative Conversion
Pyridoxine-5'-phosphate is oxidized at the C4 position — via pyridoxine-5'-phosphate oxidase in the biocatalytic route, or via a controlled chemical oxidant in the conventional route — to yield pyridoxal 5'-phosphate.
Reaction Monitoring & Endpoint Control
Conversion is monitored in real time (e.g., by HPLC/UV) to confirm reaction completion and minimize residual pyridoxine-5'-phosphate or over-oxidation byproducts.
Clarification & Biocatalyst Removal
The reaction mixture is clarified by filtration/centrifugation to remove spent enzyme, cell debris, or insoluble reaction residues from the process stream.
Purification & Crystallization
The clarified solution is purified (e.g., ion-exchange or chromatographic polishing) and subjected to controlled crystallization to obtain PLP crystals of consistent form and particle size.
Drying, Milling & Quality Release
Crystals are dried under controlled temperature and light-protected conditions, milled to target particle size, then sampled for full QC testing before batch release and packaging.
Quality Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | Light yellow to yellow crystalline powder | Conforms, light yellow crystalline powder |
| Odor | Odorless | Odorless |
| Identification (IR) | Conforms to reference spectrum | Conforms |
| Assay (HPLC) | ≥98.0% | 98.6% |
| pH (1% aqueous solution) | 4.5-6.0 | 5.2 |
| Loss on drying | ≤1.0% | 0.4% |
| Residue on ignition | ≤0.5% | 0.2% |
| Heavy metals (Pb) | ≤10 ppm | <2 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Total plate count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & mold | ≤100 CFU/g | <10 CFU/g |
| E. coli / Salmonella | Negative/25g | Negative |
Purity & Assay Method
Purity is determined by high-performance liquid chromatography (HPLC) with UV detection, typically referenced against a USP or in-house PLP working standard at approximately 325 nm, with assay reported on an as-is basis. Buyers should request the current certificate of analysis (COA) for each batch and confirm the reference standard, column/method, and acceptance criteria used, particularly if the material is intended for pharmacopeial-grade formulations.
Solubility & Formulation
PLP is soluble in water, which supports its use in aqueous-based dietary supplement formulations (liquids, effervescent tablets, oral solutions) as well as clinical nutrition applications where water solubility is required; it is generally poorly soluble in most organic solvents. Because PLP is light- and moisture-sensitive and may undergo gradual degradation in aqueous solution — particularly at extremes of pH or under prolonged UV/visible light exposure — formulators should consider light-protected packaging, controlled pH buffering, and appropriate storage conditions, and are encouraged to run compatibility and stability studies specific to their finished-product matrix.
Available Product Forms
Bulk PLP Powder
High-purity raw powder for supplement factories, research organizations, ingredient distributors, and contract manufacturers.
PLP Capsules
Custom capsule strength, shell material, bottle count, excipients, and label design for private-label brands.
PLP Tablets
Tablet formulation, granulation, compression, coating, bottle packaging, and custom labeling support.
PLP Powder Blends
PLP can be blended with complementary actives (antioxidants, other longevity ingredients, vitamins, minerals) per customer formula.
How can we confirm the exact purity and specifications before placing a bulk order?+
We provide a current certificate of analysis (COA) for every batch, including HPLC assay, appearance, heavy metals, and microbial limits. We recommend reviewing the COA against your internal specification and, for larger orders, requesting a pre-shipment sample for independent verification.
What raw material and production method is used to manufacture this PLP?+
Our PLP is manufactured starting from pyridoxine (vitamin B6) through a controlled phosphorylation and oxidation sequence, leveraging our enzymatic/biocatalytic production platform alongside conventional chemical processing steps, followed by purification and crystallization.
Is PLP soluble in water, and how should it be formulated?+
Yes, PLP is soluble in water. It is commonly formulated into aqueous-based supplements, tablets, and clinical nutrition products; given its light- and moisture-sensitivity, we recommend light-protected packaging and pH-appropriate buffering, and encourage formulation-specific stability testing.
Can we request a free sample before committing to a purchase order?+
Yes, small samples are available for qualification and testing purposes upon request; please contact our sales team with your intended application and required quantity.
Do you provide third-party lab testing or independent verification?+
In addition to our internal COA, we support submission of samples to accredited third-party laboratories chosen by the customer, and can help coordinate additional testing (e.g., heavy metals, microbial, residual solvents) as required for your market's regulatory needs.
Do you offer OEM or private-label services?+
Yes, we support OEM and private-label supply, including custom packaging, labeling, and documentation, for supplement brands, pharmaceutical formulators, and premix manufacturers.
What packaging options are available for shipment?+
Standard packaging is in light-protected, moisture-resistant containers (e.g., aluminum foil bags or drums with inner liners) in various sizes; custom packaging sizes and materials can be arranged for larger or recurring orders.
What is the minimum order quantity (MOQ) and how do we request a quotation?+
MOQ depends on packaging format and intended application; please contact our sales team with your target volume, purity requirement, and destination to receive a tailored quotation.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Batch-specific COA & full traceability on every shipment
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party laboratory testing
Documents Available
- ·Certificate of Analysis (COA)
- ·Product specification sheet
- ·Material Safety Data Sheet (MSDS)
- ·Manufacturing flow chart
- ·HPLC test report
- ·Heavy-metal test report
- ·Microbiological test report
- ·Non-GMO / allergen statement
- ·Country-of-origin documentation
- ·Packing list & commercial invoice
Certificate availability should be confirmed with our sales team before ordering.
Sample Certificate of Analysis
PathGene Biotech Co., Ltd.
南京派琪生物科技有限公司
CERTIFICATE OF ANALYSIS
Layout example — not an issued batch record
Product Name
PLP
CAS No.
54-47-7
Batch No.
PG-SAMPLE-000000
Packaging
Issued upon order confirmation
| Test Item | Specification | Result |
|---|---|---|
| Appearance | Light yellow to yellow crystalline powder | Conforms, light yellow crystalline powder |
| Odor | Odorless | Odorless |
| Identification (IR) | Conforms to reference spectrum | Conforms |
| Assay (HPLC) | ≥98.0% | 98.6% |
| pH (1% aqueous solution) | 4.5-6.0 | 5.2 |
| Loss on drying | ≤1.0% | 0.4% |
| Residue on ignition | ≤0.5% | 0.2% |
| Heavy metals (Pb) | ≤10 ppm | <2 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Total plate count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & mold | ≤100 CFU/g | <10 CFU/g |
| E. coli / Salmonella | Negative/25g | Negative |
Conclusion: Conforms to internal quality specifications
The COA issued with your order will carry the actual batch number, manufacture/test dates, and QC signature.
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Interested in PLP?
Our R&D team will respond to your inquiry or sample request within 24 hours.
