NADH
Reduced Nicotinamide Adenine Dinucleotide Disodium Salt
Key Specifications
| CAS Number | 606-68-8 |
| Purity | ≥98.0% |
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂·2Na |
| Molecular Weight | 707.40 g/mol |
| Appearance | White to off-white lyophilized powder |
| Solubility | Soluble in water |
| Storage | ≤-20°C, protect from light |
| Shelf Life | 18 months |
CAS 606-68-8
What Is NADH?
NADH disodium salt is the reduced, disodium form of nicotinamide adenine dinucleotide (NAD+), a pyridine dinucleotide coenzyme present in virtually all living cells, where it serves as the principal hydride/electron carrier in cellular redox metabolism. It is identified by CAS 606-68-8, molecular formula C21H27N7O14P2·2Na, and MW 707.40 g/mol, and occurs naturally in vivo as it cycles continuously with NAD+ during glycolysis, the citric acid cycle, and oxidative phosphorylation. Commercial bulk NADH is not built up from petrochemical feedstocks by classical organic synthesis; it is produced biotechnologically — NAD+ is generated by microbial fermentation, then converted to the reduced NADH form under controlled biocatalytic conditions, before being purified, salt-formed with sodium, and lyophilized into a stable powder suitable for supplement, research, and diagnostic-reagent applications.
Other Names
Key Features
High, Verified Purity
Assayed at ≥98.0% purity by HPLC against a reference standard, with each production batch accompanied by a certificate of analysis for buyer verification.
Synthetic Biology-Based Production
Produced on a synthetic biology and enzymatic conversion platform rather than uncontrolled extraction, giving tighter batch-to-batch consistency in yield and impurity profile.
Stable Lyophilized Powder
Supplied as a white to off-white lyophilized powder that is soluble in water, facilitating handling, weighing, and downstream formulation.
Full Contaminant Screening
Every batch is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella) to support food, supplement, and lab-reagent use.
Flexible Bulk & OEM Supply
Available in flexible bulk quantities with OEM/private-label packaging options, backed by an ISO-certified production and quality system.
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂·2Na |
| Molecular Weight | 707.40 g/mol |
| Appearance | White to off-white lyophilized powder |
| Solubility | Soluble in water |
| Storage Condition | ≤-20°C, protect from light |
| Shelf Life | 18 months |
| Grade 1 | Supplement Grade · ≥98.0% · COA |
End-Product Applications
Mechanism & Benefits
Direct NADH supplementation. Participates in energy metabolism, reduces fatigue, improves neurological function.
Packaging Options
100 g 500 g 1 kg
Lead Time
7–10 business days
Minimum Order
100 g
Custom Packaging
Custom packaging (foil bags, HDPE drums), GMP labeling, and export documentation are available. Contact us for specifics.Certifications
ISO
ISO
Manufacturing Process
Strain Fermentation
A selected microbial strain is cultured under controlled fermentation conditions to biosynthesize NAD+ as the key intermediate precursor.
Cell Harvest & Extraction
Fermentation broth is harvested and the cells are lysed to release intracellular NAD+, which is then extracted from the cellular debris.
Biocatalytic Reduction to NADH
Extracted NAD+ is converted to its reduced form, NADH, through a controlled enzymatic/biocatalytic hydride-transfer reduction step run under mild, oxygen-limited conditions to preserve the reduced cofactor.
Chromatographic Purification
The crude NADH solution is purified using ion-exchange and/or adsorption chromatography to remove residual proteins, salts, and process-related impurities.
Concentration & Salt Formation
The purified NADH solution is concentrated and reacted with a sodium source to form the stable disodium salt.
Precipitation/Crystallization
NADH disodium salt is precipitated or crystallized from the concentrate to further improve purity and remove residual mother-liquor impurities.
Lyophilization
The isolated material is freeze-dried under vacuum to yield the stable white to off-white lyophilized powder while minimizing thermal degradation of the reduced cofactor.
Milling, QC & Packaging
The lyophilized cake is milled to a uniform powder, tested against full quality specifications, and packaged under conditions that protect the product from light and moisture.
Quality Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to off-white lyophilized powder | Conforms |
| Odor | Odorless | Conforms |
| Assay (HPLC) | ≥98.0% | 98.3% |
| Loss on Drying | ≤5.0% | 3.1% |
| pH (1% aqueous solution) | 6.0–8.0 | 7.2 |
| Solubility | Clear, colorless to pale yellow solution in water (10 mg/mL) | Conforms |
| Lead (Pb) | ≤10 ppm | <5 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25 g | Negative |
| Salmonella | Negative/25 g | Negative |
Purity & Assay Method
Purity is determined by high-performance liquid chromatography (HPLC), typically with UV detection near 340 nm where the reduced dinucleotide shows characteristic absorbance, run against a qualified NADH reference standard. Because NADH is redox-sensitive and can gradually oxidize back to NAD+ under heat, light, or acidic conditions, buyers should confirm the assay method, reference standard, and storage/handling conditions used for each certificate of analysis, and request batch-specific COAs before large-volume purchase.
Solubility & Formulation
NADH disodium salt is soluble in water, forming a clear, colorless to pale yellow solution suitable for aqueous formulation work. Because the reduced form is sensitive to oxidation, heat, and light, aqueous solutions are best prepared fresh (or used shortly after reconstitution), kept at a mildly alkaline to neutral pH, protected from direct light, and stored cold once dissolved; the dry lyophilized powder itself is comparatively easier to handle and ship at ambient or refrigerated conditions. For supplement or research formulations, buffered aqueous or lyophilized single-dose formats are commonly recommended to maintain potency through the product's shelf life.
Available Product Forms
Bulk NADH Powder
High-purity raw powder for supplement factories, research organizations, ingredient distributors, and contract manufacturers.
NADH Capsules
Custom capsule strength, shell material, bottle count, excipients, and label design for private-label brands.
NADH Tablets
Tablet formulation, granulation, compression, coating, bottle packaging, and custom labeling support.
NADH Powder Blends
NADH can be blended with complementary actives (antioxidants, other longevity ingredients, vitamins, minerals) per customer formula.
How is the ≥98.0% purity of this NADH disodium salt confirmed?+
Each production batch is tested by HPLC against a qualified reference standard, and a certificate of analysis (COA) is provided with every shipment. We recommend buyers review the COA and, where required, arrange third-party verification before large orders.
How is this NADH manufactured — is it fermentation-derived or chemically synthesized?+
It is produced via a biotechnological route rather than classical chemical synthesis: NAD+ is generated by microbial fermentation and then converted to its reduced NADH form through a controlled enzymatic/biocatalytic reduction step, followed by purification, salt formation, and lyophilization.
Is NADH disodium salt water-soluble, and how should it be formulated?+
Yes, it is soluble in water. Because the reduced form is sensitive to oxidation, heat, and light, we recommend preparing solutions fresh, keeping a near-neutral pH, protecting from light, and storing dissolved material cold; the dry powder is more stable for storage and transport.
Can we request a sample before placing a bulk order?+
Yes, small samples are available on request for quality evaluation and compatibility testing prior to committing to a bulk purchase order.
Can this product undergo third-party laboratory testing?+
Yes, in addition to our internal COA for each batch, we support buyers sending samples to independent third-party laboratories for purity, heavy metal, and microbial testing.
Do you offer OEM or private-label services for this ingredient?+
Yes, we support OEM and private-label supply, including custom packaging, labeling, and documentation to meet different customer branding and regulatory requirements.
What packaging options are available for this product?+
Standard packaging is typically in moisture-proof, light-protective bulk drums or bags with an inner liner, and packaging size, unit weight, and labeling can be customized to buyer specifications.
What is the minimum order quantity (MOQ), and how can I get a quotation?+
MOQ and pricing depend on order volume, packaging format, and destination, and are provided upon request based on the buyer's specific requirements — please contact our team with your intended order quantity for a formal quotation.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Batch-specific COA & full traceability on every shipment
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party laboratory testing
Documents Available
- ·Certificate of Analysis (COA)
- ·Product specification sheet
- ·Material Safety Data Sheet (MSDS)
- ·Manufacturing flow chart
- ·HPLC test report
- ·Heavy-metal test report
- ·Microbiological test report
- ·Non-GMO / allergen statement
- ·Country-of-origin documentation
- ·Packing list & commercial invoice
Certificate availability should be confirmed with our sales team before ordering.
Sample Certificate of Analysis
PathGene Biotech Co., Ltd.
南京派琪生物科技有限公司
CERTIFICATE OF ANALYSIS
Layout example — not an issued batch record
Product Name
NADH
CAS No.
606-68-8
Batch No.
PG-SAMPLE-000000
Packaging
Issued upon order confirmation
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to off-white lyophilized powder | Conforms |
| Odor | Odorless | Conforms |
| Assay (HPLC) | ≥98.0% | 98.3% |
| Loss on Drying | ≤5.0% | 3.1% |
| pH (1% aqueous solution) | 6.0–8.0 | 7.2 |
| Solubility | Clear, colorless to pale yellow solution in water (10 mg/mL) | Conforms |
| Lead (Pb) | ≤10 ppm | <5 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25 g | Negative |
| Salmonella | Negative/25 g | Negative |
Conclusion: Conforms to internal quality specifications
The COA issued with your order will carry the actual batch number, manufacture/test dates, and QC signature.
Related Products
NRHM
Nicotinamide Riboside Hydrogen Malate
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