NAD⁺
β-Nicotinamide Adenine Dinucleotide
Key Specifications
| CAS Number | 53-84-9 |
| Purity | ≥98.0% |
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂ |
| Molecular Weight | 663.43 g/mol |
| Appearance | White to off-white powder |
| Solubility | Soluble in water |
| Storage | ≤-20°C, protect from light |
| Shelf Life | 18 months |
CAS 53-84-9
What Is NAD⁺?
β-Nicotinamide Adenine Dinucleotide (NAD⁺, CAS 53-84-9, C21H27N7O14P2, MW 663.43 g/mol) is a dinucleotide coenzyme formed by an adenine nucleotide and a nicotinamide nucleotide joined through a pyrophosphate bridge. It occurs naturally in every living cell, where it serves as the principal redox carrier in glycolysis, the TCA cycle, oxidative phosphorylation, and as a substrate for sirtuins and other NAD-consuming enzymes; it was first isolated from yeast extract in the early 1900s. Because chemical total synthesis of NAD⁺ is structurally complex and cost-prohibitive at scale, commercial bulk material is typically produced through microbial fermentation or biosynthetic/enzymatic routes using engineered yeast or bacterial strains that accumulate high intracellular NAD⁺ pools, followed by cell lysis, extraction, and chromatographic purification to the required purity grade.
Other Names
Key Features
High Assayed Purity
Each batch is assayed at ≥98.0% purity by HPLC, meeting the requirements commonly expected for dietary supplement and biochemical research applications.
Controlled Biosynthetic Production
Produced on a synthetic biology and enzymatic-conversion platform with engineered production strains and closely monitored fermentation parameters, supporting consistent batch-to-batch quality.
Consistent Powder Form
Supplied as a white to off-white powder with controlled particle size, facilitating accurate weighing, blending, and downstream formulation.
Comprehensive Contaminant Screening
Every batch is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella) under an ISO9001-based quality system.
Flexible Bulk & OEM Supply
Available in flexible bulk quantities with OEM/private-label packaging options to support brand owners, formulators, and distributors of varying order sizes.
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂ |
| Molecular Weight | 663.43 g/mol |
| Appearance | White to off-white powder |
| Solubility | Soluble in water |
| Storage Condition | ≤-20°C, protect from light |
| Shelf Life | 18 months |
| Grade 1 | Research/Industrial Grade · ≥98.0% · COA |
End-Product Applications
Mechanism & Benefits
Direct NAD⁺ supplementation. Activates sirtuins, regulates cellular metabolism and maintains cellular homeostasis.
Packaging Options
1 kg 5 kg 25 kg
Lead Time
5–7 business days
Minimum Order
1 kg
Custom Packaging
Custom packaging (foil bags, HDPE drums), GMP labeling, and export documentation are available. Contact us for specifics.Certifications
ISO
ISO
ISO9001
ISO 9001
Manufacturing Process
Strain Selection & Seed Culture
A high-yield production strain (engineered yeast or bacterium), optimized through synthetic biology approaches for elevated intracellular NAD⁺ accumulation, is activated and expanded through seed culture stages.
Controlled Fermentation
The seed culture is transferred to production bioreactors where temperature, pH, dissolved oxygen, and nutrient feeding are closely controlled to maximize intracellular NAD⁺ titer.
Cell Harvest & Disruption
Fermentation broth is harvested by centrifugation or filtration, and the cell biomass is disrupted (mechanically or enzymatically) to release intracellular NAD⁺ into solution.
Extraction
The lysate undergoes aqueous extraction under controlled thermal and pH conditions to solubilize NAD⁺ while minimizing degradation of the acid- and heat-sensitive nicotinamide-ribose bond.
Clarification & Impurity Removal
Cell debris, proteins, and nucleic acids are removed through precipitation, filtration, and decolorization steps to yield a clarified NAD⁺-containing solution.
Chromatographic Purification
Ion-exchange chromatography separates NAD⁺ from structurally related nucleotides and residual fermentation by-products to achieve the target purity level.
Concentration & Crystallization
The purified eluate is concentrated under vacuum and subjected to controlled crystallization or precipitation to isolate solid NAD⁺.
Drying, Milling & Packaging
The crystalline solid is dried under low-temperature or vacuum/freeze-drying conditions, milled and sieved to the specified particle size, then packaged under controlled humidity for shipment.
Quality Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to off-white powder | White to off-white powder |
| Odor | Odorless, characteristic | Conforms |
| Assay (HPLC) | ≥98.0% | 98.6% |
| Particle Size | Through 80 mesh, ≥95% | 97% |
| Loss on Drying | ≤8.0% | 5.1% |
| pH (1% aqueous solution) | 3.5–5.5 | 4.3 |
| Heavy Metals (as Pb) | ≤10 mg/kg | ≤2 mg/kg |
| Mercury (Hg) | ≤1 mg/kg | <0.1 mg/kg |
| Arsenic (As) | ≤2 mg/kg | <1 mg/kg |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25 g | Negative |
| Salmonella | Negative/25 g | Negative |
Purity & Assay Method
Purity is determined by high-performance liquid chromatography (HPLC) with UV detection, referencing a validated NAD⁺ standard and comparing retention time and peak area against related nucleotide impurities (e.g., NADH, AMP, nicotinamide). Buyers evaluating a supplier should confirm the specific HPLC method (column, mobile phase, detection wavelength), request the Certificate of Analysis (CoA) for the batch of interest, and, where regulatory submission is required, arrange independent third-party verification.
Solubility & Formulation
NAD⁺ is soluble in water, which makes it compatible with aqueous-based formulations such as effervescent tablets, sublingual drops, beverage powders, and injectable-grade research solutions (subject to appropriate sterility and stability studies). Because the molecule is sensitive to extremes of pH, heat, and light, formulators are generally advised to maintain solutions near neutral to slightly acidic pH, minimize prolonged heat exposure during processing, and consider protective excipients or encapsulation technologies to preserve stability through shelf life.
Available Product Forms
Bulk NAD⁺ Powder
High-purity raw powder for supplement factories, research organizations, ingredient distributors, and contract manufacturers.
NAD⁺ Capsules
Custom capsule strength, shell material, bottle count, excipients, and label design for private-label brands.
NAD⁺ Tablets
Tablet formulation, granulation, compression, coating, bottle packaging, and custom labeling support.
NAD⁺ Powder Blends
NAD⁺ can be blended with complementary actives (antioxidants, other longevity ingredients, vitamins, minerals) per customer formula.
How is the ≥98.0% purity specification confirmed for each batch?+
Each production batch is tested in-house by HPLC against a reference standard, and results are documented on a batch-specific Certificate of Analysis (CoA), which is provided with every shipment upon request.
What production method is used to manufacture this NAD⁺?+
The material is produced via a fermentation-based, biosynthetic/enzymatic process using an engineered production strain, followed by extraction and chromatographic purification, rather than through de novo chemical synthesis.
How soluble is the product, and what formulation formats does it support?+
NAD⁺ is soluble in water and is commonly used in aqueous-based formats such as tablets, drops, and powder blends; formulation guidance can be discussed based on the intended finished product.
Can we request a sample before placing a bulk order?+
Yes, small samples are generally available for quality evaluation prior to a bulk purchase; please contact the sales team to arrange sample size and shipping terms.
Do you support independent third-party testing?+
Yes, buyers are welcome to submit samples to their own third-party laboratory for independent verification of purity, heavy metals, and microbial limits in addition to the CoA provided.
Do you offer OEM or private-label services?+
Yes, OEM and private-label options are available, including customized packaging, labeling, and formulation support for brand owners and finished-product manufacturers.
What packaging options are available?+
Standard packaging typically includes food/pharma-grade drums or bags in various bulk sizes, with moisture-protective inner liners; custom packaging sizes can be arranged on request.
What is the minimum order quantity (MOQ), and how can we get a quotation?+
MOQ varies depending on order specifications and current production scheduling; please contact the sales team with your target quantity and application to receive a formal quotation.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Batch-specific COA & full traceability on every shipment
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party laboratory testing
Documents Available
- ·Certificate of Analysis (COA)
- ·Product specification sheet
- ·Material Safety Data Sheet (MSDS)
- ·Manufacturing flow chart
- ·HPLC test report
- ·Heavy-metal test report
- ·Microbiological test report
- ·Non-GMO / allergen statement
- ·Country-of-origin documentation
- ·Packing list & commercial invoice
Certificate availability should be confirmed with our sales team before ordering.
Sample Certificate of Analysis
PathGene Biotech Co., Ltd.
南京派琪生物科技有限公司
CERTIFICATE OF ANALYSIS
Layout example — not an issued batch record
Product Name
NAD⁺
CAS No.
53-84-9
Batch No.
PG-SAMPLE-000000
Packaging
Issued upon order confirmation
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to off-white powder | White to off-white powder |
| Odor | Odorless, characteristic | Conforms |
| Assay (HPLC) | ≥98.0% | 98.6% |
| Particle Size | Through 80 mesh, ≥95% | 97% |
| Loss on Drying | ≤8.0% | 5.1% |
| pH (1% aqueous solution) | 3.5–5.5 | 4.3 |
| Heavy Metals (as Pb) | ≤10 mg/kg | ≤2 mg/kg |
| Mercury (Hg) | ≤1 mg/kg | <0.1 mg/kg |
| Arsenic (As) | ≤2 mg/kg | <1 mg/kg |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25 g | Negative |
| Salmonella | Negative/25 g | Negative |
Conclusion: Conforms to internal quality specifications
The COA issued with your order will carry the actual batch number, manufacture/test dates, and QC signature.
Related Products
NRHM
Nicotinamide Riboside Hydrogen Malate
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