NAD⁺
CAS 53-84-9
Purity≥99%
NAD coenzyme material for biochemical and assay development
For B2B ingredient sourcing, request the specification, batch COA, sample, packaging and lead-time confirmation. Explore NAD+ ingredients.
Confirm oxidized NAD form, counter-ion and assay basis.
Key Specifications
| Product Positioning | NAD coenzyme material for biochemical and assay development |
| Application Scenarios | coenzyme assay development · enzyme-reaction systems · biochemical reference material |
| CAS Number | 53-84-9 |
| Purity | ≥99% |
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂ |
| Molecular Weight | 663.43 g/mol |
| Appearance | White to off-white powder |
| Solubility | Soluble in water |
| Storage | Sealed packaging, stored at cool, dry and away from light |
| Shelf Life | 18 months |
Procurement & delivery
Supply details confirmed by intended use, volume and target market
β-Nicotinamide Adenine Dinucleotide (NAD⁺, CAS 53-84-9, C21H27N7O14P2, MW 663.43 g/mol) is a dinucleotide coenzyme formed by an adenine nucleotide and a nicotinamide nucleotide joined through a pyrophosphate bridge. It occurs naturally in every living cell, where it serves as the principal redox carrier in glycolysis, the TCA cycle, oxidative phosphorylation, and as a substrate for sirtuins and other NAD-consuming enzymes; it was first isolated from yeast extract in the early 1900s. Because chemical total synthesis of NAD⁺ is structurally complex and cost-prohibitive at scale, commercial bulk material is typically produced through microbial fermentation or biosynthetic/enzymatic routes using engineered yeast or bacterial strains that accumulate high intracellular NAD⁺ pools, followed by cell lysis, extraction, and chromatographic purification to the required purity grade.
≥99% Assayed Purity
A third-party report for the tested NAD+ batch shows endotoxin ≤0.1 EU/mg. Endotoxin results are batch-specific; confirm the current lot number, test method, limit and report before qualification.
Lyophilized, Water-Soluble Powder
Supplied as lyophilized powder produced in a GMP workshop and designed for aqueous reconstitution. Confirm clarity, pH compatibility and stability in the final matrix.
High Assayed Purity
Each batch is assayed at ≥98.0% purity by HPLC, meeting the requirements commonly expected for dietary supplement and biochemical research applications.
Controlled Biosynthetic Production
Produced on a synthetic biology and enzymatic-conversion platform with engineered production strains and closely monitored fermentation parameters, supporting consistent batch-to-batch quality.
Consistent Powder Form
Supplied as a white to off-white powder with controlled particle size, facilitating accurate weighing, blending, and downstream formulation.
Comprehensive Contaminant Screening
Every batch is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella), with results independently verified by third-party laboratories.
Flexible Bulk & OEM Supply
Available in flexible bulk quantities with OEM/private-label packaging options to support brand owners, formulators, and distributors of varying order sizes.
Test items, specifications and batch results are shown here; request the formal COA through the card below.
Batch No.
PG01-260423
Test date
2026-04-24
Specification
In-house
Pack size
25 kg/carton
Storage
Sealed packaging, stored at cool, dry and away from light
Conclusion
Conforms
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to off-white crystalline powder | Complies |
| Identification | HPLC main peak retention time consistent with reference | Complies |
| Purity | ≥99.0% | 99.6% |
| Assay (dry basis) | ≥98.0% | 98.6% |
| Loss on drying | ≤8% | 7.2% |
| pH | 2.0–4.0 | 3.3 |
| Ethanol residue | ≤1% | ≤0.01% |
| Lead (Pb) | ≤0.1 ppm | Complies |
| Mercury (Hg) | ≤0.1 ppm | Complies |
| Cadmium (Cd) | ≤0.2 ppm | Complies |
| Arsenic (As) | ≤0.1 ppm | Complies |
| Total microbial count | ≤500 CFU/g | <10 |
| Coliform | ≤0.92 MPN/g | Complies |
| Mold and yeast | ≤50 CFU/g | Complies |
| Staphylococcus aureus | 0/25 g | Complies |
| Salmonella | 0/25 g | Complies |
| Bulk density | — | 0.72 g/ml |
| Tapped density | — | 0.87 g/ml |
| Particle size | NLT 95% pass 60 mesh | Conforms |
This page shows a batch-data summary only. No direct COA file link is provided; request the signed document through the form below.
Dietary supplements
Enzyme catalysis
Pharmaceutical and API development
Diagnostic reagent raw materials
NAD⁺ is a central redox coenzyme, while PQQ is a redox-active compound studied in mitochondrial biogenesis. Together they can be evaluated as a formulation direction for energy metabolism and oxidative-stress research.
Evidence: This is based on mechanistic and review evidence; it is not proof of clinically established synergy. Compatibility, stability and finished-dosage-form testing are required.Source
NAD⁺ is a substrate for sirtuins, while resveratrol is studied in SIRT1/AMPK and oxidative-stress signaling. The pair may be evaluated as a cellular energy and stress-signaling research direction.
Evidence: Evidence is mainly mechanistic and from reviews, not a finished-product or human efficacy conclusion. Dose, solubility and light stability should be assessed during development.Source
TMG can participate in homocysteine remethylation through the BHMT pathway, providing a one-carbon and methyl-donor research angle. This is a formulation hypothesis to test, not a required pairing.
Evidence: TMG's methyl-donor role is documented, but direct synergy between NAD⁺ and TMG in finished products has not been established. Target population, dose and regulatory suitability require evaluation.Source
Literature-Supported Research Directions
NAD⁺/NADH are central redox coenzymes in glycolysis, the TCA cycle and oxidative phosphorylation. Research focuses on their dynamics in cellular energy metabolism and redox homeostasis.
Citation: Bender et al., Cell Metabolism (2016): review of NAD⁺ metabolism, bioenergetics and signaling.View source
NAD⁺ also serves as a substrate for sirtuins, PARPs and CD38, linking protein deacetylation, DNA-damage responses, inflammatory signaling and NAD⁺ homeostasis.
Citation: Covarrubias et al., Nature Reviews Molecular Cell Biology (2021): NAD⁺ metabolism and cellular processes during ageing.View source
Current work examines how NAD⁺ synthesis, consumption and compartmentalization influence metabolism, DNA repair, mitochondrial homeostasis and stress responses, primarily in biochemical and cellular models.
Citation: Katsyuba et al., Nature Metabolism (2020): pathophysiologic mechanisms and therapeutic potential of NAD⁺ metabolism.View source
Note: For ingredient R&D and scientific information; findings depend on model, dose, formulation and population and do not constitute a treatment claim or efficacy guarantee.
Lead Time
3–5business days
Standard products are generally in stock; confirm the exact lead time with us.
Minimum Order
1 kg
Samples, pilot trials, and commercial volumes are supported.
Foil Pouch
Sealed, moisture-protective inner packaging
Fiber Drum
Standard outer packaging for bulk transport
Bulk Shipping
Palletized and stretch-wrapped for protection
Ambient storage: foil pouch + fiber drum; temperature controlled: foil pouch + insulated box/carton. Confirm by product.
Custom packaging: Custom foil bags or HDPE drums, GMP label printing, and export documentation are available. Contact us for a quote.Images are packaging illustrations.
Certifications
Strain Selection & Seed Culture
A high-yield production strain (engineered yeast or bacterium), optimized through synthetic biology approaches for elevated intracellular NAD⁺ accumulation, is activated and expanded through seed culture stages.
Controlled Fermentation
The seed culture is transferred to production bioreactors where temperature, pH, dissolved oxygen, and nutrient feeding are closely controlled to maximize intracellular NAD⁺ titer.
Cell Harvest & Disruption
Fermentation broth is harvested by centrifugation or filtration, and the cell biomass is disrupted (mechanically or enzymatically) to release intracellular NAD⁺ into solution.
Extraction
The lysate undergoes aqueous extraction under controlled thermal and pH conditions to solubilize NAD⁺ while minimizing degradation of the acid- and heat-sensitive nicotinamide-ribose bond.
Clarification & Impurity Removal
Cell debris, proteins, and nucleic acids are removed through precipitation, filtration, and decolorization steps to yield a clarified NAD⁺-containing solution.
Chromatographic Purification
Ion-exchange chromatography separates NAD⁺ from structurally related nucleotides and residual fermentation by-products to achieve the target purity level.
Concentration & Crystallization
The purified eluate is concentrated under vacuum and subjected to controlled crystallization or precipitation to isolate solid NAD⁺.
Drying, Milling & Packaging
The crystalline solid is dried under low-temperature or vacuum/freeze-drying conditions, milled and sieved to the specified particle size, then packaged under controlled humidity for shipment.
Purity is determined by high-performance liquid chromatography (HPLC) with UV detection, referencing a validated NAD⁺ standard and comparing retention time and peak area against related nucleotide impurities (e.g., NADH, AMP, nicotinamide). Buyers evaluating a supplier should confirm the specific HPLC method (column, mobile phase, detection wavelength), request the Certificate of Analysis (CoA) for the batch of interest, and, where regulatory submission is required, arrange independent third-party verification.
NAD⁺ is readily soluble in water and is supplied as a lyophilized powder for aqueous reconstitution. Evaluate pH, concentration, light exposure and hold time in the final matrix. A low-endotoxin result does not establish sterility, pyrogen-free status, injectable suitability or finished-product regulatory compliance.
No. The third-party endotoxin result of ≤0.1 EU/mg applies only to the tested batch. It does not by itself demonstrate sterility, pyrogen-free status, injectable suitability or finished-product regulatory compliance. The buyer must validate the final intended use.
Request the current batch COA together with the applicable third-party endotoxin report, including batch number, test method, reported limit/result and report date. Review these documents against your own research or diagnostic-reagent raw-material requirements.
It is supplied as lyophilized powder produced in a GMP workshop and is readily soluble in water. Reconstitution conditions, including concentration, pH, clarity and stability, should be confirmed in the target formulation.
Each production batch is tested in-house by HPLC against a reference standard, and results are documented on a batch-specific Certificate of Analysis (CoA), which is provided with every shipment upon request.
The material is produced via a fermentation-based, biosynthetic/enzymatic process using an engineered production strain, followed by extraction and chromatographic purification, rather than through de novo chemical synthesis.
NAD⁺ is soluble in water and is commonly used in aqueous-based formats such as tablets, drops, and powder blends; formulation guidance can be discussed based on the intended finished product.
Yes, small samples are generally available for quality evaluation prior to a bulk purchase; please contact the sales team to arrange sample size and shipping terms.
Yes, buyers are welcome to submit samples to their own third-party laboratory for independent verification of purity, heavy metals, and microbial limits in addition to the CoA provided.
Yes, OEM and private-label options are available, including customized packaging, labeling, and formulation support for brand owners and finished-product manufacturers.
Standard packaging typically includes food/pharma-grade drums or bags in various bulk sizes, with moisture-protective inner liners; custom packaging sizes can be arranged on request.
MOQ varies depending on order specifications and current production scheduling; please contact the sales team with your target quantity and application to receive a formal quotation.
Documents are subject to product and batch availability; third-party reports are provided only when applicable.
Quality documents
The specification sheet defines acceptance limits. The COA shows actual batch results, which may vary by batch. Third-party reports are provided only for products or batches that have been independently tested.
Stable acceptance criteria · Available on request
Actual test data · Issued with each shipment
Independent test record · Available by product/batch
Technical Resources
Selling NMN, NR or NAD+ in Australia? Compare TGA listing conditions, NRC food-use permissions and the questions to resolve before sourcing ingredients.
A procurement-focused guide to verifying a low-endotoxin NAD+ powder claim: lot-matched reports, test method, interference controls, packaging and the boundary between raw-material qualification and sterile-product release.
A procurement checklist for NAD+ bulk material covering identity, assay, related substances, elemental impurities, residual solvents, microbiology, and batch COA.
Related Products
NMN Powder (β-Nicotinamide Mononucleotide)
Nicotinamide Riboside Chloride Powder (NR/NRC)
NRHM Powder (Nicotinamide Riboside Hydrogen Malate)
NMNH Powder (Reduced Nicotinamide Mononucleotide)
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Alex Huang · Mon–Fri 9am–6pm CST
