NADPH
Reduced Nicotinamide Adenine Dinucleotide Phosphate Tetrasodium Salt
Key Specifications
| CAS Number | 2646-71-1 |
| Purity | ≥95.0% |
| Molecular Formula | C₂₁H₂₆N₇O₁₇P₃·4Na |
| Molecular Weight | 833.33 g/mol |
| Appearance | White to light yellow lyophilized powder |
| Solubility | Soluble in water |
| Storage | ≤-20°C, inert atmosphere |
| Shelf Life | 12 months |
CAS 2646-71-1
What Is NADPH?
NADPH tetrasodium salt (CAS 2646-71-1; C21H26N7O17P3·4Na, MW 833.33) is the sodium salt form of reduced nicotinamide adenine dinucleotide phosphate, a universal biological cofactor found in all living cells where it supplies reducing equivalents for anabolic pathways such as fatty acid and nucleotide biosynthesis and cellular antioxidant defense. Because chemical total synthesis of this labile dinucleotide is impractical at scale, commercial bulk material is produced biotechnologically: a fermentation-derived NAD+/NADP+ precursor is enzymatically phosphorylated and then reduced by dehydrogenase-based biocatalytic systems, after which the reduced cofactor is purified, converted to its tetrasodium salt, and lyophilized into a stable powder for research and industrial use.
Other Names
Key Features
Verified High Purity
Purity of ≥95.0% confirmed by HPLC against a reference standard, with batch-specific certificates of analysis available on request.
Controlled Biosynthetic Production
Produced via a traceable enzymatic conversion route from fermentation-derived precursors, with process parameters monitored at every stage to ensure batch-to-batch consistency.
Stable Lyophilized Form
Supplied as a fine white to light yellow lyophilized powder, freely soluble in water, formulated for ease of handling and extended shelf life when stored properly.
Rigorous Contaminant Testing
Each lot is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella) to meet biochemical-grade requirements.
Flexible Bulk & OEM Supply
Available in flexible pack sizes from gram-scale research quantities to bulk industrial volumes, with OEM/private-label packaging options for distributors and formulators.
| Molecular Formula | C₂₁H₂₆N₇O₁₇P₃·4Na |
| Molecular Weight | 833.33 g/mol |
| Appearance | White to light yellow lyophilized powder |
| Solubility | Soluble in water |
| Storage Condition | ≤-20°C, inert atmosphere |
| Shelf Life | 12 months |
| Grade 1 | Research Grade · ≥95.0% · COA |
End-Product Applications
Mechanism & Benefits
Participates in cellular oxidoreduction reactions. Used in biochemical experiments and enzyme activity detection.
Packaging Options
10 g 50 g 100 g
Lead Time
7–10 business days
Minimum Order
10 g
Custom Packaging
Custom packaging (foil bags, HDPE drums), GMP labeling, and export documentation are available. Contact us for specifics.Certifications
ISO9001
ISO 9001
Manufacturing Process
Microbial Fermentation for Precursor Supply
A production strain (e.g., engineered yeast or bacteria) is fermented under controlled conditions to generate nicotinamide adenine dinucleotide (NAD+)-related precursor biomass, which is extracted and clarified.
Enzymatic Phosphorylation to NADP+
NAD kinase catalyzes ATP-dependent phosphorylation of the NAD+ precursor at the 2'-hydroxyl of the adenosine ribose, converting it to NADP+.
Biocatalytic Reduction to NADPH
NADP+ is reduced to NADPH using a dehydrogenase-based cofactor regeneration system (e.g., glucose dehydrogenase or formate dehydrogenase), under controlled pH and temperature to preserve the redox-sensitive dihydronicotinamide ring.
Reaction Termination and Clarification
The enzymatic reaction is quenched, and biomass, residual protein, and cell debris are removed by centrifugation and ultrafiltration.
Chromatographic Purification
Ion-exchange and/or reverse-phase chromatography separates NADPH from unreacted NADP+/NAD+, ADP, and other reaction by-products.
Salt Formation and Crystallization
The purified NADPH is converted to its tetrasodium salt form by controlled sodium hydroxide/sodium salt addition, followed by concentration and crystallization.
Freeze-Drying (Lyophilization)
The crystallized product is freeze-dried under vacuum, shielded from light and oxygen, to yield a stable lyophilized powder.
Quality Control and Packaging
Finished material undergoes HPLC purity assay, appearance and moisture testing, and microbial/heavy-metal screening before being packaged under desiccated, light-protected conditions.
Quality Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to light yellow lyophilized powder | Conforms |
| Odor | Odorless | Conforms |
| Purity (HPLC) | ≥95.0% | 96.5% |
| Identification (UV spectrum) | Characteristic absorption, conforms to reference standard | Conforms |
| Physical form | Fine, uniform lyophilized powder | Conforms |
| Loss on drying (moisture) | ≤8.0% | 5.4% |
| Heavy metals (Pb) | ≤10 ppm | <5 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Total plate count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25g | Negative |
| Salmonella | Negative/25g | Negative |
Purity & Assay Method
Purity is assessed by high-performance liquid chromatography (HPLC) with UV detection, comparing retention time and peak area against a certified NADPH reference standard; the characteristic 340 nm absorbance of the reduced dihydronicotinamide ring is also used to confirm that the material remains in its reduced (not oxidized to NADP+) state. Buyers should request the batch-specific HPLC chromatogram and certificate of analysis, and confirm the assay method and acceptance criteria used before large-scale procurement.
Solubility & Formulation
NADPH tetrasodium salt is freely soluble in water, making it convenient to prepare aqueous stock solutions for enzymatic assays and formulation work; it is not appreciably soluble in most organic solvents. Because the reduced cofactor is redox- and pH-sensitive—prone to oxidation and faster degradation under acidic conditions or prolonged exposure to light and air—solutions are best prepared fresh in a mildly alkaline buffer (approximately pH 7-8), kept cold and shielded from light, and used promptly or aliquoted and stored frozen to avoid repeated freeze-thaw cycles.
Available Product Forms
Bulk NADPH Powder
High-purity raw powder for supplement factories, research organizations, ingredient distributors, and contract manufacturers.
NADPH Capsules
Custom capsule strength, shell material, bottle count, excipients, and label design for private-label brands.
NADPH Tablets
Tablet formulation, granulation, compression, coating, bottle packaging, and custom labeling support.
NADPH Powder Blends
NADPH can be blended with complementary actives (antioxidants, other longevity ingredients, vitamins, minerals) per customer formula.
How is the ≥95.0% purity of this NADPH tetrasodium salt confirmed?+
Each batch is tested by HPLC against a certified reference standard, and a certificate of analysis (COA) with the actual assay result is provided with every shipment; the COA and raw chromatogram can be shared for review before order confirmation.
How is this NADPH tetrasodium salt manufactured — is it fermentation-derived, chemically synthesized, or extracted?+
It is produced by a biotechnological route: a fermentation-derived NAD+/NADP+ precursor is enzymatically phosphorylated and then reduced by a dehydrogenase-based cofactor regeneration system, followed by chromatographic purification, salt formation, and lyophilization. Chemical total synthesis is not used, as the reduced dinucleotide is not practical to build from scratch at industrial scale.
How should I prepare and store solutions of this product?+
The tetrasodium salt is freely soluble in water. We recommend preparing solutions fresh in a mildly alkaline buffer (pH ~7-8), protecting from light, and storing at -20°C in single-use aliquots to minimize oxidation and freeze-thaw degradation.
Can I request a sample before placing a bulk order?+
Yes, small-quantity samples are available for evaluation. Please contact our sales team with your intended application so we can recommend the appropriate sample size and pack format.
Is third-party testing or an independent COA available?+
In addition to our in-house COA for every batch, we can arrange third-party laboratory testing (e.g., purity, heavy metals, microbial limits) upon request for buyers who require independent verification.
Do you offer OEM or private-label supply for this product?+
Yes, we support OEM/private-label packaging and labeling for distributors, formulators, and brand owners, including customized pack sizes and documentation to match your specifications.
What packaging options are available?+
Standard packaging ranges from small research-grade vials (e.g., grams) to bulk industrial packaging (kilograms), typically in moisture-proof, light-protected containers with desiccant; custom packaging can be arranged for larger orders.
What is the minimum order quantity (MOQ), and how can I get a quotation?+
MOQ varies by pack size and intended use, from gram-scale research orders to bulk industrial volumes. Please contact our sales team with your required quantity, purity grade, and delivery location for a tailored quotation.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Batch-specific COA & full traceability on every shipment
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party laboratory testing
Documents Available
- ·Certificate of Analysis (COA)
- ·Product specification sheet
- ·Material Safety Data Sheet (MSDS)
- ·Manufacturing flow chart
- ·HPLC test report
- ·Heavy-metal test report
- ·Microbiological test report
- ·Non-GMO / allergen statement
- ·Country-of-origin documentation
- ·Packing list & commercial invoice
Certificate availability should be confirmed with our sales team before ordering.
Sample Certificate of Analysis
PathGene Biotech Co., Ltd.
南京派琪生物科技有限公司
CERTIFICATE OF ANALYSIS
Layout example — not an issued batch record
Product Name
NADPH
CAS No.
2646-71-1
Batch No.
PG-SAMPLE-000000
Packaging
Issued upon order confirmation
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to light yellow lyophilized powder | Conforms |
| Odor | Odorless | Conforms |
| Purity (HPLC) | ≥95.0% | 96.5% |
| Identification (UV spectrum) | Characteristic absorption, conforms to reference standard | Conforms |
| Physical form | Fine, uniform lyophilized powder | Conforms |
| Loss on drying (moisture) | ≤8.0% | 5.4% |
| Heavy metals (Pb) | ≤10 ppm | <5 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Total plate count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25g | Negative |
| Salmonella | Negative/25g | Negative |
Conclusion: Conforms to internal quality specifications
The COA issued with your order will carry the actual batch number, manufacture/test dates, and QC signature.
Related Products
NRHM
Nicotinamide Riboside Hydrogen Malate
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