NADPH
CAS 2646-71-1
Purity≥95.0%
Reduced NADP coenzyme for redox-enzyme research
For B2B ingredient sourcing, request the specification, batch COA, sample, packaging and lead-time confirmation. Explore NAD+ ingredients.
Confirm reduced-form proportion and storage conditions.
Key Specifications
| Product Positioning | Reduced NADP coenzyme for redox-enzyme research |
| Application Scenarios | reductase reactions · enzymatic synthesis · redox detection systems |
| CAS Number | 2646-71-1 |
| Purity | ≥95.0% |
| Molecular Formula | C₂₁H₂₆N₇O₁₇P₃·4Na |
| Molecular Weight | 833.33 g/mol |
| Appearance | White to light yellow lyophilized powder |
| Solubility | Soluble in water |
| Storage | ≤-20°C, inert atmosphere |
| Shelf Life | 12 months |
Procurement & delivery
Supply details confirmed by intended use, volume and target market
NADPH tetrasodium salt (CAS 2646-71-1; C21H26N7O17P3·4Na, MW 833.33) is the sodium salt form of reduced nicotinamide adenine dinucleotide phosphate, a universal biological cofactor found in all living cells where it supplies reducing equivalents for anabolic pathways such as fatty acid and nucleotide biosynthesis and cellular antioxidant defense. Because chemical total synthesis of this labile dinucleotide is impractical at scale, commercial bulk material is produced biotechnologically: a fermentation-derived NAD+/NADP+ precursor is enzymatically phosphorylated and then reduced by dehydrogenase-based biocatalytic systems, after which the reduced cofactor is purified, converted to its tetrasodium salt, and lyophilized into a stable powder for research and industrial use.
Verified High Purity
Purity of ≥95.0% confirmed by HPLC against a reference standard, with batch-specific certificates of analysis available on request.
Controlled Biosynthetic Production
Produced via a traceable enzymatic conversion route from fermentation-derived precursors, with process parameters monitored at every stage to ensure batch-to-batch consistency.
Stable Lyophilized Form
Supplied as a fine white to light yellow lyophilized powder, freely soluble in water, formulated for ease of handling and extended shelf life when stored properly.
Rigorous Contaminant Testing
Each lot is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella) to meet biochemical-grade requirements.
Flexible Bulk & OEM Supply
Available in flexible pack sizes from gram-scale research quantities to bulk industrial volumes, with OEM/private-label packaging options for distributors and formulators.
Test items, specifications and batch results are shown here; request the formal COA through the card below.
Batch No.
PGNADPH-260625
Test date
2026-07-01
Specification
In-house
Pack size
1 g、5 g/瓶
Storage
≤-20°C, inert atmosphere
Conclusion
Conforms
| Test Item | Specification | Result |
|---|---|---|
| Appearance | Yellow or pale yellow powder | Complies |
| Identification | HPLC main peak retention time consistent with reference | Complies |
| Solubility | Dissolves easily in water | Complies |
| Purity | ≥93.0% | 98.28% |
| Water (KF) | ≤8% | 1.66% |
| pH | 7.0–11.0 | 10.0 |
This page shows a batch-data summary only. No direct COA file link is provided; request the signed document through the form below.
Enzyme catalysis
Diagnostic reagent raw materials
G6PD with glucose-6-phosphate can form an NADPH-regeneration module for continuous reducing-equivalent supply in reductase reactions and biomanufacturing.
Evidence: Suitable for enzyme catalysis and process R&D; substrate consumption, by-products, recycling efficiency and stability require validation.Source
NADPH can be paired with NADPH-dependent oxidoreductases to screen chiral reductions, hydroxylations and other reductive biotransformations.
Evidence: This is a biocatalysis R&D direction and not a nutrition or therapeutic efficacy statement.Source
Literature-Supported Research Directions
NADPH supplies reducing power for lipid, nucleotide and antioxidant systems and is a common cofactor-regeneration target in metabolic engineering and biomanufacturing. Research focuses on supply-demand balance, regeneration efficiency and side-reaction control.
Citation: Review of NADPH metabolic engineering and regeneration (2024).View source
NADPH can be used in fluorescent, colorimetric and enzyme-coupled assays for oxidoreductase activity, substrate conversion and diagnostic-method development. Linearity, background and cofactor stability require platform-specific validation.
Citation: Research on rapid fluorescent NADPH detection.View source
The NADP⁺/NADPH ratio can be used to study cellular reducing state, cofactor regeneration and oxidative-stress responses in research and diagnostic-method development.
Citation: Methods research on NAD(P)(H) redox-cofactor analysis.View source
Note: For ingredient R&D and scientific information; findings depend on model, dose, formulation and population and do not constitute a treatment claim or efficacy guarantee.
Lead Time
3–5business days
Standard products are generally in stock; confirm the exact lead time with us.
Minimum Order
10 g
Samples, pilot trials, and commercial volumes are supported.
Foil Pouch
Sealed, moisture-protective inner packaging
Insulated Box + Carton
For products requiring temperature-controlled transport
Bulk Shipping
Palletized and stretch-wrapped for protection
Ambient storage: foil pouch + fiber drum; temperature controlled: foil pouch + insulated box/carton. Confirm by product.
Custom packaging: Custom foil bags or HDPE drums, GMP label printing, and export documentation are available. Contact us for a quote.Images are packaging illustrations.
Certifications
Microbial Fermentation for Precursor Supply
A production strain (e.g., engineered yeast or bacteria) is fermented under controlled conditions to generate nicotinamide adenine dinucleotide (NAD+)-related precursor biomass, which is extracted and clarified.
Enzymatic Phosphorylation to NADP+
NAD kinase catalyzes ATP-dependent phosphorylation of the NAD+ precursor at the 2'-hydroxyl of the adenosine ribose, converting it to NADP+.
Biocatalytic Reduction to NADPH
NADP+ is reduced to NADPH using a dehydrogenase-based cofactor regeneration system (e.g., glucose dehydrogenase or formate dehydrogenase), under controlled pH and temperature to preserve the redox-sensitive dihydronicotinamide ring.
Reaction Termination and Clarification
The enzymatic reaction is quenched, and biomass, residual protein, and cell debris are removed by centrifugation and ultrafiltration.
Chromatographic Purification
Ion-exchange and/or reverse-phase chromatography separates NADPH from unreacted NADP+/NAD+, ADP, and other reaction by-products.
Salt Formation and Crystallization
The purified NADPH is converted to its tetrasodium salt form by controlled sodium hydroxide/sodium salt addition, followed by concentration and crystallization.
Freeze-Drying (Lyophilization)
The crystallized product is freeze-dried under vacuum, shielded from light and oxygen, to yield a stable lyophilized powder.
Quality Control and Packaging
Finished material undergoes HPLC purity assay, appearance and moisture testing, and microbial/heavy-metal screening before being packaged under desiccated, light-protected conditions.
Purity is assessed by high-performance liquid chromatography (HPLC) with UV detection, comparing retention time and peak area against a certified NADPH reference standard; the characteristic 340 nm absorbance of the reduced dihydronicotinamide ring is also used to confirm that the material remains in its reduced (not oxidized to NADP+) state. Buyers should request the batch-specific HPLC chromatogram and certificate of analysis, and confirm the assay method and acceptance criteria used before large-scale procurement.
NADPH tetrasodium salt is freely soluble in water, making it convenient to prepare aqueous stock solutions for enzymatic assays and formulation work; it is not appreciably soluble in most organic solvents. Because the reduced cofactor is redox- and pH-sensitive—prone to oxidation and faster degradation under acidic conditions or prolonged exposure to light and air—solutions are best prepared fresh in a mildly alkaline buffer (approximately pH 7-8), kept cold and shielded from light, and used promptly or aliquoted and stored frozen to avoid repeated freeze-thaw cycles.
Each batch is tested by HPLC against a certified reference standard, and a certificate of analysis (COA) with the actual assay result is provided with every shipment; the COA and raw chromatogram can be shared for review before order confirmation.
It is produced by a biotechnological route: a fermentation-derived NAD+/NADP+ precursor is enzymatically phosphorylated and then reduced by a dehydrogenase-based cofactor regeneration system, followed by chromatographic purification, salt formation, and lyophilization. Chemical total synthesis is not used, as the reduced dinucleotide is not practical to build from scratch at industrial scale.
The tetrasodium salt is freely soluble in water. We recommend preparing solutions fresh in a mildly alkaline buffer (pH ~7-8), protecting from light, and storing at -20°C in single-use aliquots to minimize oxidation and freeze-thaw degradation.
Yes, small-quantity samples are available for evaluation. Please contact our sales team with your intended application so we can recommend the appropriate sample size and pack format.
In addition to our in-house COA for every batch, we can arrange third-party laboratory testing (e.g., purity, heavy metals, microbial limits) upon request for buyers who require independent verification.
Yes, we support OEM/private-label packaging and labeling for distributors, formulators, and brand owners, including customized pack sizes and documentation to match your specifications.
Standard packaging ranges from small research-grade vials (e.g., grams) to bulk industrial packaging (kilograms), typically in moisture-proof, light-protected containers with desiccant; custom packaging can be arranged for larger orders.
MOQ varies by pack size and intended use, from gram-scale research orders to bulk industrial volumes. Please contact our sales team with your required quantity, purity grade, and delivery location for a tailored quotation.
Documents are subject to product and batch availability; third-party reports are provided only when applicable.
Quality documents
The specification sheet defines acceptance limits. The COA shows actual batch results, which may vary by batch. Third-party reports are provided only for products or batches that have been independently tested.
Stable acceptance criteria · Available on request
Actual test data · Issued with each shipment
Technical Resources
Related Products
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NAD+ Powder (β-Nicotinamide Adenine Dinucleotide)
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NRHM Powder (Nicotinamide Riboside Hydrogen Malate)
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Alex Huang · Mon–Fri 9am–6pm CST
