NADP⁺
CAS 24292-60-2
Purity≥97.0%
Phosphorylated NAD coenzyme for enzyme-system research
Nicotinamide Adenine Dinucleotide Phosphate Disodium Salt Powder (CAS 24292-60-2), specification ≥97.0%. Oxidized NADP disodium salt, supplied separately from NADP monosodium and reduced NADPH tetrasodium. Batch COA and evaluation samples are available. MOQ: 100 g. NAD+ ingredients
Quote / sample & delivery
Key Specifications
| Product Positioning | Phosphorylated NAD coenzyme for enzyme-system research |
| Application Scenarios | NADP-dependent enzyme systems · phosphate-cofactor assays · biochemical formulation research |
| CAS Number | 24292-60-2 |
| Purity | ≥97.0% |
| Molecular Formula | C₂₁H₂₆N₇O₁₇P₃·2Na |
| Molecular Weight | 787.37 g/mol |
| Appearance | White to off-white powder |
| Solubility | Soluble in water |
| Storage | ≤-20°C, protect from light |
| Shelf Life | 18 months |
Procurement & delivery
Supply details confirmed by intended use, volume and target market
Nicotinamide adenine dinucleotide phosphate disodium salt (NADP+ disodium salt, CAS 24292-60-2) is the oxidized, water-soluble sodium form of NADP+, a dinucleotide coenzyme (C21H26N7O17P3·2Na, MW 787.37 g/mol) composed of an adenylic acid unit and a nicotinamide ribonucleotide unit joined by a pyrophosphate bridge, with an extra phosphate group on the 2'-position of the adenosine ribose that distinguishes it from NAD+. NADP+/NADPH occurs in virtually all living cells, where it serves as the principal electron carrier for reductive biosynthesis (fatty acid, cholesterol, and nucleotide synthesis) and for maintaining cellular antioxidant defense systems. Commercial bulk material is not isolated directly from natural sources; it is manufactured via a biocatalytic route in which microbially derived NAD+ is enzymatically phosphorylated by NAD kinase, then purified, converted to the sodium salt, and crystallized and dried into a stable powder. This product is NADP+ disodium salt (CAS 24292-60-2). NADP monosodium salt (CAS 1184-16-3) is a separate salt specification of the oxidized cofactor; NADPH is its reduced form. Match the salt identity and assay basis to the intended method when comparing these materials.
High-Purity, Batch-Verified Assay
Every batch is assayed by HPLC to confirm purity of ≥97.0%, with a certificate of analysis issued per lot so buyers can verify potency before use in sensitive enzymatic or research applications.
Controlled Enzymatic Production Route
Produced on our synthetic biology and enzymatic conversion platform via NAD kinase-catalyzed phosphorylation of microbially derived NAD+, with tightly controlled reaction, purification, and crystallization parameters for consistent lot-to-lot quality.
Consistent White to Off-White Powder
Supplied as a free-flowing white to off-white powder with uniform particle size, facilitating accurate weighing, dissolution, and downstream formulation handling.
Rigorous Contaminant Testing
Each batch is screened for heavy metals (Pb, As, Hg, Cd) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella) to ensure the material meets food- and lab-grade safety expectations.
Flexible Bulk and OEM Supply
Available in flexible bulk quantities with customizable packaging, labeling, and OEM/private-label options to support both trial orders and long-term commercial supply agreements.
Test items, specifications and results for the listed batch are shown here. View or request a COA in the quality documents section.
Batch No.
PGNADP2-260416
Test date
2026-04-28
Specification
In-house
Pack size
1 kg/carton
Storage
≤-20°C, protect from light
Conclusion
Conforms
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to pale yellow powder | Complies |
| Identification | HPLC main peak retention time consistent with reference | Complies |
| State of solution | Clear liquid | Complies |
| Purity | ≥95.0% | 98.6% |
| Assay | ≥95.0% | 97.5% |
| Water (KF) | ≤10% | 7.5% |
| pH | 2.0–5.0 | 3.7 |
View an available COA in the quality documents section. Check the document for its product form and batch; request a signed COA for your purchasing or shipment batch separately.
Enzyme catalysis
Diagnostic reagent raw materials
NADP⁺ disodium and monosodium are distinct supply forms; specify counter-ions, water and assay basis.
98.6%
Purity
97.5%
Assay
7.5%
Water (KF)
PGNADP2-260416 · Results apply to this lot; interpret purity, assay and water on their respective bases.
Composition candidates, processing sequences and tests support pilot design. Confirm candidate excipients and processes using this material in the actual finished formulation.
Application examples follow the materials, formats and findings actually studied. Combination studies, single-ingredient studies, commercial precedents and development candidates are distinguished.
NADP⁺ participates in G6PD-catalysed NADPH generation; human G6PD also has structural NADP⁺ binding, making enzyme source relevant.
Show research and formulation insights +Hide details −Combination / selection rationale: NADP⁺ participates in G6PD-catalysed NADPH generation; human G6PD also has structural NADP⁺ binding, making enzyme source relevant.
Published evidence / source details: A 2022 structural/biochemical study distinguished catalytic and structural NADP⁺ and examined human-G6PD conformation and catalysis, not arbitrary industrial-regeneration performance.
Development insights: Prepare molarity from the supplied disodium composition and assay basis. Screen NADP⁺, G6P and enzyme levels; record 340-nm initial rates, blanks, matrix recovery and NADPH feedback with enzyme-source documentation.
Evidence scope: Cofactor-level evidence, not validation of every disodium lot or diagnostic product.
2022 · G6PD structural/catalytic NADP⁺ · DOI 10.1073/pnas.2119695119 ↗GDH/glucose can reduce starting NADP⁺ to NADPH for a consuming reductase, rather than relying on a large stoichiometric NADPH charge.
Show research and formulation insights +Hide details −Combination / selection rationale: GDH/glucose can reduce starting NADP⁺ to NADPH for a consuming reductase, rather than relying on a large stoichiometric NADPH charge.
Published evidence / source details: A 1992 study coupled dihydrofolate reductase with Gluconobacter scleroides GDH, using 2 mM NADP and 200 mM dihydrofolate and completing conversion under its reported conditions.
Development insights: Screen cofactor loading and regenerating/consuming enzyme balance, measuring substrate/product, glucose consumption and pH drift. Convert millimolar targets using the supplied salt composition rather than a fixed mass recipe.
Evidence scope: The study does not establish equivalence of this disodium grade; changed enzymes and substrates require reevaluation.
1992 · DHFR/GDH cofactor regeneration · DOI 10.1271/bbb.56.701 ↗Literature-Supported Research Directions
NADP⁺ and NADPH form a redox pair in cellular and enzyme systems, supporting research into reducing power, lipid/nucleotide biosynthesis and oxidative-stress balance.
Citation: Sun et al. NADP+/NADPH in Metabolism and its Relation to Cardiovascular Pathologies. Current medicinal chemistry (2025). PMID: 38549538.View source
NADP⁺ is used to study cofactor recognition, catalytic efficiency and redox directionality in NADP-dependent enzymes, supporting enzyme engineering and biomanufacturing screens.
Citation: Capone et al. Re-engineering the discrimination between the oxidized coenzymes NAD+ and NADP+ in clostridial glutamate dehydrogenase and a thorough reappraisal of the coenzyme specificity of the wild-type enzyme. The FEBS journal (2011). PMID: 21564547.View source
NADP⁺ can serve as a cofactor or reference material in NAD(P)(H) quantitation, dehydrogenase assays and diagnostic reaction systems. Performance depends on method, purity and batch validation.
Citation: Lu et al. Extraction and Quantitation of Nicotinamide Adenine Dinucleotide Redox Cofactors. Antioxidants & redox signaling (2018). PMID: 28497978.View source
Note: For ingredient R&D and scientific information; findings depend on model, dose, formulation and population and do not constitute a treatment claim or efficacy guarantee.
Lead Time
3–5business days
Standard products are generally in stock; confirm the exact lead time with us.
Minimum Order
100 g
Samples, pilot trials, and commercial volumes are supported.
Foil Pouch
Sealed, moisture-protective inner packaging
Insulated Box + Carton
For products requiring temperature-controlled transport
Bulk Shipping
Palletized and stretch-wrapped for protection
Ambient storage: foil pouch + fiber drum; temperature controlled: foil pouch + insulated box/carton. Confirm by product.
Custom packaging: Custom foil bags or HDPE drums, GMP label printing, and export documentation are available. Contact us for a quote.Images are packaging illustrations.
Certifications
Microbial Fermentation of NAD+ Precursor
An engineered microbial strain is fermented under controlled conditions to biosynthesize nicotinamide adenine dinucleotide (NAD+), the direct substrate for NADP+ production.
NAD+ Extraction and Primary Purification
Fermentation broth is clarified and NAD+ is extracted and partially purified through cell lysis, filtration, and initial chromatographic separation.
Enzymatic Phosphorylation (NAD Kinase)
Purified NAD+ is converted to NADP+ through biocatalytic phosphorylation using NAD kinase, with ATP or inorganic polyphosphate serving as the phosphate donor under an ATP-regeneration or poly(P)-driven system.
Reaction Termination and Clarification
The enzymatic reaction is quenched and the mixture is clarified to remove residual enzyme, biomass, and unreacted substrates.
Chromatographic Purification
NADP+ is isolated from the reaction mixture using ion-exchange chromatography to separate it from NAD+, ADP, phosphate salts, and other reaction by-products.
Sodium Salt Formation and Crystallization
The purified NADP+ fraction is converted to its disodium salt form under controlled pH and ionic conditions and crystallized to achieve the desired form and particle characteristics.
Concentration and Drying
The crystallized product is concentrated and dried, typically by vacuum or freeze-drying, to obtain a stable, low-moisture powder while minimizing thermal degradation of the labile dinucleotide structure.
Milling, Blending, and Packaging
The dried material is milled to target particle size, blended for lot homogeneity, and packaged under controlled humidity in moisture-barrier containers.
Purity is determined by reverse-phase HPLC with UV detection (typically 254-260 nm), comparing sample peak area against a reference NADP+ standard on a dry-weight basis. Because NADP+ is sensitive to pH extremes, light, and elevated temperature, buyers should request the lot-specific certificate of analysis and confirm the HPLC method, reference standard, and storage conditions used, especially if the material will be re-tested with in-house or third-party equipment.
NADP+ disodium salt is readily soluble in water, forming a clear, colorless to pale yellow solution suitable for use in enzymatic assay buffers, cell-free reaction systems, and research-grade formulations. Aqueous solutions are best prepared fresh or stored refrigerated and protected from light, as NADP+ can gradually degrade under prolonged exposure to heat, low pH, or UV light; for buffered applications, a mildly alkaline to neutral pH (around 7-8) generally supports better solution stability.
Yes. Each production lot is tested in-house by HPLC and accompanied by a certificate of analysis covering purity, appearance, pH, loss on drying, heavy metals, and microbial limits, which we share with customers prior to or at shipment.
It is produced via a biocatalytic route on our enzymatic conversion platform: NAD+ is first obtained through microbial fermentation, then enzymatically phosphorylated to NADP+ using NAD kinase, followed by purification, sodium salt formation, and crystallization. It is not chemically synthesized from petrochemical feedstocks nor extracted directly from plant or animal tissue.
It is readily soluble in water, forming a clear solution. Because NADP+ is sensitive to heat, low pH, and prolonged light exposure, we recommend preparing solutions fresh, storing them refrigerated and protected from light, and working within a near-neutral to mildly alkaline pH range for best stability.
Yes, small trial samples are available on request so you can verify purity, solubility, and performance in your own application before committing to a bulk purchase order.
Yes. In addition to our in-house certificate of analysis, we support customers sending samples to accredited third-party laboratories for independent verification of purity, heavy metals, and microbial limits.
Yes, we offer OEM and private-label options, including custom packaging sizes, labeling, and documentation to align with your brand and regulatory requirements.
NADP disodium powder pack options are 100 g, 500 g, 1 kg. Special inner-packaging, labeling and transport requirements are agreed separately for the order.
The raw-material MOQ for NADP disodium powder is 100 g, with pack options of 100 g, 500 g, 1 kg. Select this grade separately from NADP monosodium and reduced NADPH.
Prepare the target molarity from the supplied disodium composition and appropriate assay basis. Specify enzyme source, G6P, buffer and readout; human G6PD structural-binding research does not establish every assay or regeneration process. Compare NADPH formation with blanks and verify matrix recovery and linearity in the intended method.
Documents are subject to product and batch availability; third-party reports are provided only when applicable.
Quality documents
The specification sheet defines acceptance limits. The COA shows actual batch results, which may vary by batch. Third-party reports are provided only for products or batches that have been independently tested.
Stable acceptance criteria · Available on request
Actual test data · Check the COA for the applicable batch
Technical Resources
Related Products
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β-Nicotinamide Adenine Dinucleotide (NAD+) Powder
Nicotinamide Riboside Chloride Powder
Nicotinamide Riboside Hydrogen Malate Powder
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