NMNH
Reduced Nicotinamide Mononucleotide
Key Specifications
| CAS Number | 108347-85-9 |
| Purity | ≥95.0% |
| Molecular Formula | C₁₁H₁₆N₂O₈P |
| Molecular Weight | 335.23 g/mol |
| Appearance | White to light yellow powder |
| Solubility | Soluble in water |
| Storage | ≤-20°C, inert atmosphere |
| Shelf Life | 18 months |
CAS 108347-85-9
What Is NMNH?
Reduced Nicotinamide Mononucleotide (NMNH, CAS 108347-85-9) is the dihydro (reduced) form of nicotinamide mononucleotide, a nucleotide that sits directly on the NAD+/NADH biosynthetic pathway. Its molecular formula is C11H16N2O8P with a molecular weight of 335.23 g/mol. Unlike its oxidized counterpart NMN, NMNH is not known to occur in appreciable amounts in common foods or tissues and is essentially a specialty biochemical obtained through targeted production rather than extraction. Commercial-scale material is typically manufactured via a biocatalytic route: a fermentation-derived, engineered enzyme from the Nudix pyrophosphatase family is used to selectively convert NADH into NMNH and AMP under mild aqueous reaction conditions, after which the product is purified by chromatography, concentrated under oxygen-limited conditions to protect its reduced state, and dried into a stable powder.
Other Names
Key Features
High-Purity, HPLC-Verified
Every batch is assayed at ≥95.0% purity by HPLC, ensuring consistent potency for both research and finished-product applications.
Enzymatic Biosynthesis, Tightly Controlled
Produced on a synthetic biology and enzymatic conversion platform with defined reaction, purification, and drying parameters for reliable batch-to-batch consistency.
Stable White to Light-Yellow Powder
Supplied as a free-flowing white to light yellow powder, packaged under oxygen-limited conditions to protect the reduced (NADH-type) structure during storage and transport.
Full Contaminant Screening
Each lot is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella) under an ISO-certified quality system.
Flexible Bulk & OEM Supply
Available in bulk quantities with support for OEM/private-label formulation, custom packaging sizes, and tailored documentation for supplement and research customers worldwide.
| Molecular Formula | C₁₁H₁₆N₂O₈P |
| Molecular Weight | 335.23 g/mol |
| Appearance | White to light yellow powder |
| Solubility | Soluble in water |
| Storage Condition | ≤-20°C, inert atmosphere |
| Shelf Life | 18 months |
| Grade 1 | Research Grade · ≥95.0% · COA |
End-Product Applications
Mechanism & Benefits
Direct NADH precursor. Rapidly replenishes cellular energy, reduces fatigue and improves endurance.
Packaging Options
100 g 500 g 1 kg
Lead Time
7–10 business days
Minimum Order
100 g
Custom Packaging
Custom packaging (foil bags, HDPE drums), GMP labeling, and export documentation are available. Contact us for specifics.Certifications
ISO
ISO
Manufacturing Process
Biocatalyst Preparation via Fermentation
An engineered microbial strain expressing a Nudix-family NADH pyrophosphatase is cultivated by controlled fermentation, and the enzyme is harvested and prepared as the process biocatalyst.
Substrate (NADH) Preparation
Pharmaceutical-grade NADH, itself derived from fermentation-based NAD+ biosynthesis, is prepared as the starting substrate for the enzymatic conversion step.
Enzymatic Biotransformation
The purified pyrophosphatase selectively hydrolyzes the pyrophosphate bond of NADH under controlled pH and temperature in an aqueous reaction system, yielding NMNH and AMP in near-equimolar amounts.
Reaction Monitoring & Conversion Optimization
In-process HPLC monitoring tracks substrate conversion and byproduct formation, with reaction parameters adjusted to maximize NMNH yield while minimizing re-oxidation to NMN.
Chromatographic Separation
The reaction mixture is separated by ion-exchange and/or reverse-phase chromatography to isolate NMNH from AMP, residual NADH, and other process-related impurities.
Oxygen-Limited Concentration & Crystallization
The purified NMNH fraction is concentrated under low-oxygen, temperature-controlled conditions to prevent oxidative reversion to NMN, then crystallized or precipitated.
Drying & Micronization
Material is freeze-dried or vacuum-dried under inert gas protection, then milled and sieved to the target particle size for a free-flowing powder.
Quality Control & Oxygen-Protective Packaging
Finished powder is assayed by HPLC and tested for heavy metals and microbial limits, then packed under nitrogen or vacuum-sealed conditions to preserve the reduced form.
Quality Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to light yellow powder | Conforms |
| Odor | Characteristic, no foreign odor | Conforms |
| Assay (HPLC) | ≥95.0% | 96.5% |
| Loss on Drying | ≤5.0% | 2.3% |
| Particle Size | 100% through 80 mesh | Conforms |
| Heavy Metals (Total) | ≤10 ppm | <10 ppm |
| Lead (Pb) | ≤1 ppm | <1 ppm |
| Mercury (Hg) | ≤0.1 ppm | <0.1 ppm |
| Cadmium (Cd) | ≤0.3 ppm | <0.3 ppm |
| Arsenic (As) | ≤1 ppm | <1 ppm |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25 g | Negative |
| Salmonella | Negative/25 g | Negative |
Purity & Assay Method
Purity is determined by reverse-phase HPLC with UV detection, comparing sample retention time and peak area against a qualified NMNH reference standard. Because NMNH is redox-sensitive and can slowly revert toward NMN under heat, light, or oxygen exposure, buyers should request a batch-specific certificate of analysis, confirm the assay date relative to shipment, and where relevant verify results with their own or a third-party laboratory before formulation.
Solubility & Formulation
NMNH is soluble in water, making it compatible with aqueous-based formulations such as powder blends, effervescent tablets, sublingual drops, and beverage premixes. Because the reduced form is more prone to oxidation than NMN, formulators are advised to minimize prolonged exposure to heat, light, and dissolved oxygen, consider near-neutral pH buffering, and evaluate the use of common antioxidant excipients or inert-gas processing to help preserve stability through manufacturing and shelf life.
Available Product Forms
Bulk NMNH Powder
High-purity raw powder for supplement factories, research organizations, ingredient distributors, and contract manufacturers.
NMNH Capsules
Custom capsule strength, shell material, bottle count, excipients, and label design for private-label brands.
NMNH Tablets
Tablet formulation, granulation, compression, coating, bottle packaging, and custom labeling support.
NMNH Powder Blends
NMNH can be blended with complementary actives (antioxidants, other longevity ingredients, vitamins, minerals) per customer formula.
What purity and specifications does your NMNH meet, and can this be confirmed before purchase?+
Our standard NMNH is manufactured to ≥95.0% purity by HPLC, with full specifications covering appearance, assay, loss on drying, heavy metals, and microbial limits. A current certificate of analysis is provided with every shipment, and pre-shipment COAs can be shared for review before you place an order.
How is NMNH produced, and what is the starting raw material?+
NMNH is produced through a biocatalytic process built on our synthetic biology and enzymatic conversion platform. A Nudix-family pyrophosphatase enzyme selectively converts NADH (itself derived from fermentation-based NAD+ biosynthesis) into NMNH under mild aqueous conditions, followed by chromatographic purification, oxygen-limited concentration, and drying.
Is NMNH soluble in water, and how should we handle it in formulation?+
Yes, NMNH is soluble in water and works well in aqueous formulations. Because the reduced form is oxidation-sensitive, we recommend minimizing exposure to heat, light, and oxygen during processing and storage; our technical team can share general handling guidance on request.
Can we request a sample before committing to a bulk order?+
Yes, small samples are available for quality evaluation, formulation trials, or third-party testing before you commit to a bulk purchase. Please contact our sales team with your intended application to arrange sample shipment.
Do you provide third-party test reports for NMNH?+
Every batch is tested in-house under our ISO-certified quality system, and we can coordinate third-party laboratory testing (e.g., for purity, heavy metals, or microbial limits) upon request, generally at the buyer's cost unless otherwise agreed.
Do you offer OEM or private-label services for NMNH-based products?+
Yes, we support OEM and private-label programs, including custom blends, capsule/tablet/powder formats, and branded packaging, for customers looking to bring finished NMNH products to market.
What packaging options are available for bulk NMNH?+
Standard bulk packaging uses oxygen-limited, moisture-protective containers (such as vacuum-sealed or nitrogen-flushed foil-lined drums or bags) in common trade quantities, with custom packaging sizes and labeling available on request.
What is the minimum order quantity (MOQ), and how can we get a quotation?+
MOQ varies by packaging format and intended use; please contact our sales team with your target quantity, application, and destination to receive a tailored quotation and lead time.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Batch-specific COA & full traceability on every shipment
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party laboratory testing
Documents Available
- ·Certificate of Analysis (COA)
- ·Product specification sheet
- ·Material Safety Data Sheet (MSDS)
- ·Manufacturing flow chart
- ·HPLC test report
- ·Heavy-metal test report
- ·Microbiological test report
- ·Non-GMO / allergen statement
- ·Country-of-origin documentation
- ·Packing list & commercial invoice
Certificate availability should be confirmed with our sales team before ordering.
Sample Certificate of Analysis
PathGene Biotech Co., Ltd.
南京派琪生物科技有限公司
CERTIFICATE OF ANALYSIS
Layout example — not an issued batch record
Product Name
NMNH
CAS No.
108347-85-9
Batch No.
PG-SAMPLE-000000
Packaging
Issued upon order confirmation
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to light yellow powder | Conforms |
| Odor | Characteristic, no foreign odor | Conforms |
| Assay (HPLC) | ≥95.0% | 96.5% |
| Loss on Drying | ≤5.0% | 2.3% |
| Particle Size | 100% through 80 mesh | Conforms |
| Heavy Metals (Total) | ≤10 ppm | <10 ppm |
| Lead (Pb) | ≤1 ppm | <1 ppm |
| Mercury (Hg) | ≤0.1 ppm | <0.1 ppm |
| Cadmium (Cd) | ≤0.3 ppm | <0.3 ppm |
| Arsenic (As) | ≤1 ppm | <1 ppm |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative/25 g | Negative |
| Salmonella | Negative/25 g | Negative |
Conclusion: Conforms to internal quality specifications
The COA issued with your order will carry the actual batch number, manufacture/test dates, and QC signature.
Related Products
NRHM
Nicotinamide Riboside Hydrogen Malate
Interested in NMNH?
Our R&D team will respond to your inquiry or sample request within 24 hours.
