Spermidine
Spermidine Trihydrochloride
Key Specifications
| CAS Number | 334-50-9 |
| Purity | ≥99.0% |
| Molecular Formula | C₇H₁₉N₃·3HCl |
| Molecular Weight | 254.62 g/mol |
| Appearance | White crystalline powder |
| Solubility | Freely soluble in water |
| Storage | 2–8°C, protect from moisture |
| Shelf Life | 24 months |
CAS 334-50-9
What Is Spermidine?
Spermidine trihydrochloride (CAS 334-50-9) is the stable, water-soluble hydrochloride salt of spermidine, a naturally occurring aliphatic polyamine (chemical formula C7H19N3·3HCl, molecular weight 254.62 g/mol) present in virtually every living cell, from bacteria to humans. It is produced endogenously in mammalian tissue and also occurs at relatively high concentrations in foods such as wheat germ, soybeans, aged cheese, natto, and certain mushrooms, though both dietary and endogenous levels are reported to decline with age. Commercial bulk spermidine trihydrochloride is typically obtained by extraction and purification from plant sources such as wheat germ, by chemical synthesis from putrescine, or, increasingly, by microbial fermentation using metabolically engineered production strains combined with enzymatic conversion steps, followed by chromatographic purification and crystallization as the trihydrochloride salt to reach food- or pharmaceutical-grade purity.
Other Names
Key Features
High-Purity, HPLC-Verified
Each batch is assayed at ≥99.0% purity by HPLC, with a certificate of analysis provided to confirm identity and content against the reference standard.
Controlled Bio-Manufacturing Process
Produced under PathGene's synthetic biology and enzymatic production platform, using engineered production strains and tightly monitored fermentation parameters for consistent batch-to-batch quality.
Stable Crystalline Form
Supplied as a free-flowing white crystalline powder, freely soluble in water, that is easy to handle, dose, and incorporate into downstream formulations.
Rigorous Contaminant Screening
Every lot is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits, keeping impurities well within specification for food- and supplement-grade use.
Flexible Bulk & OEM Supply
Available in bulk quantities from trial-order to full-container volumes, with OEM/private-label formulation, custom packaging, and documentation support.
| Molecular Formula | C₇H₁₉N₃·3HCl |
| Molecular Weight | 254.62 g/mol |
| Appearance | White crystalline powder |
| Solubility | Freely soluble in water |
| Storage Condition | 2–8°C, protect from moisture |
| Shelf Life | 24 months |
| Grade 1 | Pharmaceutical Grade · ≥99.0% · COA |
End-Product Applications
Mechanism & Benefits
Activates cellular autophagy, delays cellular senescence, improves memory and protects cardiovascular health.
Packaging Options
1 kg 5 kg 25 kg
Lead Time
5–7 business days
Minimum Order
1 kg
Custom Packaging
Custom packaging (foil bags, HDPE drums), GMP labeling, and export documentation are available. Contact us for specifics.Certifications
ISO
ISO
ISO9001
ISO 9001
Manufacturing Process
Strain Engineering & Seed Culture
A metabolically engineered microbial production strain, optimized along the ornithine/putrescine-to-spermidine biosynthetic pathway, is revived and expanded through seed culture stages under strict aseptic control.
Fed-Batch Fermentation
Seed culture is transferred into production bioreactors where temperature, pH, dissolved oxygen, and carbon-source feed rate are precisely controlled to maximize intracellular spermidine accumulation.
Enzymatic Conversion
A targeted enzymatic conversion step (leveraging spermidine synthase-type activity) drives efficient conversion of the putrescine precursor pool into spermidine, improving overall yield and selectivity.
Cell Harvest & Lysis
Fermentation broth is separated by centrifugation or membrane filtration, and cells are lysed to release intracellular spermidine into solution for downstream recovery.
Extraction & Chromatographic Purification
Clarified extract is passed through ion-exchange and/or chromatographic resins to selectively capture the polyamine while removing residual proteins, sugars, and fermentation salts.
Salt Formation & Crystallization
The purified spermidine solution is treated with hydrochloric acid under controlled conditions to form the trihydrochloride salt, which is then crystallized and recrystallized to raise purity and remove trace impurities.
Drying & Milling
Wet crystals are vacuum-dried to the target moisture level and milled to a uniform particle size, yielding the final white crystalline powder.
Quality Control & Packaging
Finished material undergoes full QC testing (HPLC assay, heavy metals, microbial limits) before being packaged under low-humidity conditions to preserve stability during storage and transport.
Quality Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White crystalline powder | Complies, white crystalline powder |
| Odor | Odorless to faint characteristic odor | Complies |
| Assay (HPLC) | ≥99.0% | 99.3% |
| Loss on Drying | ≤1.0% | 0.4% |
| Residue on Ignition | ≤0.5% | 0.2% |
| pH (1% aqueous solution) | 4.0–6.0 | 5.1 |
| Solubility | Freely soluble in water | Complies |
| Heavy Metals (Pb) | ≤10 ppm | <5 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli / Salmonella | Negative/25g | Negative |
Purity & Assay Method
Purity is determined by high-performance liquid chromatography (HPLC) with UV detection against a qualified reference standard, reported on an anhydrous, salt-corrected basis. Because spermidine trihydrochloride is hygroscopic and its purity value depends heavily on the analytical method and reference standard used, buyers are encouraged to request the batch-specific certificate of analysis, confirm the HPLC method parameters, and, where required, arrange independent third-party verification of both assay and moisture content.
Solubility & Formulation
Spermidine trihydrochloride is freely soluble in water, forming clear, colorless to faintly yellow solutions well suited to aqueous-based formulations such as capsules, compressed tablets (with suitable excipients), effervescent powders, and liquid or beverage-format supplements. As the salt is hygroscopic and the polyamine can be sensitive to prolonged air and light exposure, formulators are advised to store the material under sealed, low-humidity conditions, minimize processing time under open-air conditions, and consider mild acidification, encapsulation, or coating technologies to protect stability and help mask its characteristic odor in finished products.
Available Product Forms
Bulk Spermidine Powder
High-purity raw powder for supplement factories, research organizations, ingredient distributors, and contract manufacturers.
Spermidine Capsules
Custom capsule strength, shell material, bottle count, excipients, and label design for private-label brands.
Spermidine Tablets
Tablet formulation, granulation, compression, coating, bottle packaging, and custom labeling support.
Spermidine Powder Blends
Spermidine can be blended with complementary actives (antioxidants, other longevity ingredients, vitamins, minerals) per customer formula.
What purity level do you guarantee, and how is it verified?+
We guarantee ≥99.0% purity by HPLC assay, with a batch-specific certificate of analysis (CoA) issued for every shipment. Customers are welcome to request additional documentation or arrange independent lab verification.
How is your spermidine trihydrochloride manufactured?+
It is produced via PathGene's synthetic biology and enzymatic production platform: an engineered microbial strain is fermented to generate spermidine, which undergoes enzymatic conversion, chromatographic purification, and crystallization as the trihydrochloride salt. This differs from wheat-germ extraction and offers more consistent, scalable quality.
Is spermidine trihydrochloride soluble in water, and can it be used in liquid formulations?+
Yes, it is freely soluble in water, making it suitable for liquid or beverage-format products in addition to capsules, tablets, and powders. We can advise on basic formulation considerations such as pH and stability upon request.
Can we request a sample before placing a bulk order?+
Yes, small samples with a full CoA and safety data sheet are available for evaluation purposes. Please contact us with your intended application to arrange a sample shipment.
Do you support third-party or independent laboratory testing?+
Yes, we fully support independent verification. Samples can be shipped directly to a testing laboratory of your choice, and we can provide the reference standard information needed for comparison.
Can you provide OEM or private-label services?+
Yes, we offer OEM/private-label support including custom formulation ratios, finished-dose forms (capsule, tablet, powder), and customer-branded packaging and labeling.
What packaging options are available?+
Standard packaging includes 1 kg and 5 kg foil bags with desiccant and 25 kg fiber drums; custom packaging sizes and formats can be arranged for larger or specialized orders.
What is the minimum order quantity, and how do we get a quotation?+
Minimum order quantities are flexible, starting from small trial orders up to full-container volumes. Please contact us with your required quantity, target application, and destination to receive a formal quotation.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Batch-specific COA & full traceability on every shipment
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party laboratory testing
Documents Available
- ·Certificate of Analysis (COA)
- ·Product specification sheet
- ·Material Safety Data Sheet (MSDS)
- ·Manufacturing flow chart
- ·HPLC test report
- ·Heavy-metal test report
- ·Microbiological test report
- ·Non-GMO / allergen statement
- ·Country-of-origin documentation
- ·Packing list & commercial invoice
Certificate availability should be confirmed with our sales team before ordering.
Sample Certificate of Analysis
PathGene Biotech Co., Ltd.
南京派琪生物科技有限公司
CERTIFICATE OF ANALYSIS
Layout example — not an issued batch record
Product Name
Spermidine
CAS No.
334-50-9
Batch No.
PG-SAMPLE-000000
Packaging
Issued upon order confirmation
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White crystalline powder | Complies, white crystalline powder |
| Odor | Odorless to faint characteristic odor | Complies |
| Assay (HPLC) | ≥99.0% | 99.3% |
| Loss on Drying | ≤1.0% | 0.4% |
| Residue on Ignition | ≤0.5% | 0.2% |
| pH (1% aqueous solution) | 4.0–6.0 | 5.1 |
| Solubility | Freely soluble in water | Complies |
| Heavy Metals (Pb) | ≤10 ppm | <5 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli / Salmonella | Negative/25g | Negative |
Conclusion: Conforms to internal quality specifications
The COA issued with your order will carry the actual batch number, manufacture/test dates, and QC signature.
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Interested in Spermidine?
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