Spermidine
CAS 334-50-9
Purity≥99.0%
Polyamine research material for cell and nutrition formulations
Spermidine Trihydrochloride Powder (CAS 334-50-9), specification ≥99.0%. Crystalline spermidine trihydrochloride, distinct from spermidine free base and spermidine-containing botanical extracts. Batch COA and evaluation samples are available. MOQ: 1 kg. healthy-aging ingredients
Quote / sample & delivery
Key Specifications
| Product Positioning | Polyamine research material for cell and nutrition formulations |
| Application Scenarios | polyamine cell research · nutrition capsule screening · aqueous formulation work |
| CAS Number | 334-50-9 |
| Purity | ≥99.0% |
| Molecular Formula | C₇H₁₉N₃·3HCl |
| Molecular Weight | 254.62 g/mol |
| Appearance | White crystalline powder |
| Solubility | Freely soluble in water |
| Storage | 2–8°C, protect from moisture |
| Shelf Life | 24 months |
Procurement & delivery
Supply details confirmed by intended use, volume and target market
Spermidine trihydrochloride (CAS 334-50-9) is the stable, water-soluble hydrochloride salt of spermidine, a naturally occurring aliphatic polyamine (chemical formula C7H19N3·3HCl, molecular weight 254.62 g/mol) present in virtually every living cell, from bacteria to humans. It is produced endogenously in mammalian tissue and also occurs at relatively high concentrations in foods such as wheat germ, soybeans, aged cheese, natto, and certain mushrooms, though both dietary and endogenous levels are reported to decline with age. Commercial bulk spermidine trihydrochloride is typically obtained by extraction and purification from plant sources such as wheat germ, by chemical synthesis from putrescine, or, increasingly, by microbial fermentation using metabolically engineered production strains combined with enzymatic conversion steps, followed by chromatographic purification and crystallization as the trihydrochloride salt to reach food- or pharmaceutical-grade purity.
High-Purity, HPLC-Verified
Each batch is assayed at ≥99.0% purity by HPLC, with a certificate of analysis provided to confirm identity and content against the reference standard.
Non-GMO, Allergen-Free & Gluten-Free
Documentation for non-GMO, allergen-free and gluten-free status can be provided for applicable projects. Final claim use should be confirmed against the target-market requirements and the supporting batch documentation.
High Active Content for Compact Formats
Compared with wheat-germ extracts standardized to 1% spermidine, this ≥99.0% trihydrochloride material provides more than 50 times higher spermidine content on a raw-material comparison, supporting lower inclusion levels and compact dosage-form development. Final dosage should be verified in the finished formulation.
Rigorous Contaminant Screening
Every lot is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits, keeping impurities well within specification for food- and supplement-grade use.
Flexible Bulk & OEM Supply
Available in bulk quantities from trial-order to full-container volumes, with OEM/private-label formulation, custom packaging, and documentation support.
Test items, specifications and results for the listed batch are shown here. View or request a COA in the quality documents section.
Batch No.
PGSPD-260518
Test date
2026-05-19
Specification
In-house
Pack size
25kg/carton
Storage
2–8°C, protect from moisture
Conclusion
Conforms
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White crystalline powder | Complies, white crystalline powder |
| Odor | Odorless to faint characteristic odor | Complies |
| Assay (HPLC) | ≥99.0% | 99.3% |
| Loss on Drying | ≤1.0% | 0.4% |
| Residue on Ignition | ≤0.5% | 0.2% |
| pH (1% aqueous solution) | 4.0–6.0 | 5.1 |
| Solubility | Freely soluble in water | Complies |
| Heavy Metals (Pb) | ≤10 ppm | <5 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Total Plate Count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli / Salmonella | Negative/25g | Negative |
Request a reviewed COA through the quality documents section and confirm its product form and batch.
Hard capsules
Calculate against actual trihydrochloride identity and spermidine moiety; free-base and salt masses differ.
99.3%
Assay (HPLC)
PGSPD-260518 · Results apply to this lot; interpret purity, assay and water on their respective bases.
Composition candidates, processing sequences and tests support pilot design. Confirm candidate excipients and processes using this material in the actual finished formulation.
Application examples follow the materials, formats and findings actually studied. Combination studies, single-ingredient studies, commercial precedents and development candidates are distinguished.
A defined polyamine salt suits low-loading solids, requiring separate control of salt mass, spermidine equivalents and unit uniformity.
Show research and formulation insights +Hide details −Combination / selection rationale: A defined polyamine salt suits low-loading solids, requiring separate control of salt mass, spermidine equivalents and unit uniformity.
Published evidence / source details: A 2024 placebo-controlled trial in 37 men aged 50–70 studied high-purity spermidine trihydrochloride for up to 28 days. Clinical safety measures and serum/urine polyamines showed no substantial changes.
Development insights: Define declaration by salt or spermidine moiety and correct loading using an appropriate mass assay. Geometrically premix with filler and test transfer segregation, unit assay, moisture and barrier packaging; study loading is not a new-product intake recommendation.
Evidence scope: Short-term tolerance/exposure evidence, not longevity, autophagy efficacy or long-term safety.
2024 · Supplementation of spermidine at 40 mg/day has minimal effects on circulating polyamines: An exploratory double-blind randomized controlled trial in older men. · DOI 10.1016/j.nutres.2024.09.012 ↗High-purity salt and plant extracts have different carrier loads; design capsule fills around measured particle size and density.
Show research and formulation insights +Hide details −Combination / selection rationale: High-purity salt and plant extracts have different carrier loads; design capsule fills around measured particle size and density.
Published evidence / source details: The 2024 trial did not show large circulating-polyamine rises, supporting separate measurement of supplied content and biological endpoints rather than inferring exposure from purity.
Development insights: Screen candidate fillers such as mannitol/MCC with ingredient, carrier, premix and capsule samples. Assay blend positions, filling stages and stored polyamines rather than relying on total nitrogen or chloride.
Evidence scope: Development design, not validation of this premix or arbitrary carriers.
2024 · Supplementation of spermidine at 40 mg/day has minimal effects on circulating polyamines: An exploratory double-blind randomized controlled trial in older men. · DOI 10.1016/j.nutres.2024.09.012 ↗Literature-Supported Research Directions
Spermidine is an endogenous polyamine involved in cellular growth and stress responses. Autophagy-enhancing activity is a major research basis for healthy-aging and cellular-maintenance studies.
Citation: Wirth et al., Cortex (2018): spermidine-rich supplementation and memory performance in older adults at risk for dementia.View source
A phase IIa pilot trial reported a signal for improved mnemonic discrimination, while the longer 12-month SmartAge trial did not significantly change the primary memory endpoint. The mixed results support further dose, formulation and population research rather than a definitive cognitive claim.
Citation: Schwarz et al., JAMA Network Open (2022): 12-month randomized clinical trial of spermidine supplementation.View source
Note: For ingredient R&D and scientific information; findings depend on model, dose, formulation and population and do not constitute a treatment claim or efficacy guarantee.
Lead Time
3–5business days
Standard products are generally in stock; confirm the exact lead time with us.
Minimum Order
1 kg
Samples, pilot trials, and commercial volumes are supported.
Foil Pouch
Sealed, moisture-protective inner packaging
Insulated Box + Carton
For products requiring temperature-controlled transport
Bulk Shipping
Palletized and stretch-wrapped for protection
Ambient storage: foil pouch + fiber drum; temperature controlled: foil pouch + insulated box/carton. Confirm by product.
Custom packaging: Custom foil bags or HDPE drums, GMP label printing, and export documentation are available. Contact us for a quote.Images are packaging illustrations.
Certifications
Strain Engineering & Seed Culture
A metabolically engineered microbial production strain, optimized along the ornithine/putrescine-to-spermidine biosynthetic pathway, is revived and expanded through seed culture stages under strict aseptic control.
Fed-Batch Fermentation
Seed culture is transferred into production bioreactors where temperature, pH, dissolved oxygen, and carbon-source feed rate are precisely controlled to maximize intracellular spermidine accumulation.
Enzymatic Conversion
A targeted enzymatic conversion step (leveraging spermidine synthase-type activity) drives efficient conversion of the putrescine precursor pool into spermidine, improving overall yield and selectivity.
Cell Harvest & Lysis
Fermentation broth is separated by centrifugation or membrane filtration, and cells are lysed to release intracellular spermidine into solution for downstream recovery.
Extraction & Chromatographic Purification
Clarified extract is passed through ion-exchange and/or chromatographic resins to selectively capture the polyamine while removing residual proteins, sugars, and fermentation salts.
Salt Formation & Crystallization
The purified spermidine solution is treated with hydrochloric acid under controlled conditions to form the trihydrochloride salt, which is then crystallized and recrystallized to raise purity and remove trace impurities.
Drying & Milling
Wet crystals are vacuum-dried to the target moisture level and milled to a uniform particle size, yielding the final white crystalline powder.
Quality Control & Packaging
Finished material undergoes full QC testing (HPLC assay, heavy metals, microbial limits) before being packaged under low-humidity conditions to preserve stability during storage and transport.
Purity is determined by high-performance liquid chromatography (HPLC) with UV detection against a qualified reference standard, reported on an anhydrous, salt-corrected basis. Because spermidine trihydrochloride is hygroscopic and its purity value depends heavily on the analytical method and reference standard used, buyers are encouraged to request the batch-specific certificate of analysis, confirm the HPLC method parameters, and, where required, arrange independent third-party verification of both assay and moisture content.
Spermidine trihydrochloride is freely soluble in water, forming clear, colorless to faintly yellow solutions well suited to aqueous-based formulations such as capsules, compressed tablets (with suitable excipients), effervescent powders, and liquid or beverage-format supplements. As the salt is hygroscopic and the polyamine can be sensitive to prolonged air and light exposure, formulators are advised to store the material under sealed, low-humidity conditions, minimize processing time under open-air conditions, and consider mild acidification, encapsulation, or coating technologies to protect stability and help mask its characteristic odor in finished products.
Store spermidine trihydrochloride powder at 2–8°C and protect it from moisture. Its listed shelf life is 24 months; the lot label and documents identify the applicable expiry date. Reseal after opening and arrange transport temperature and packaging for the supplied material.
Spermidine trihydrochloride is the salt identified by CAS 334-50-9, with an HPLC purity specification of ≥99.0%. Free-base spermidine and spermidine-containing plant extracts have different compositions and assay bases. Verify salt identity and the COA calculation basis before comparing active-component cost or formulation quantities.
We guarantee ≥99.0% purity by HPLC assay, with a batch-specific certificate of analysis (CoA) issued for every shipment. Customers are welcome to request additional documentation or arrange independent lab verification.
It is produced via PathGene's synthetic biology and enzymatic production platform: an engineered microbial strain is fermented to generate spermidine, which undergoes enzymatic conversion, chromatographic purification, and crystallization as the trihydrochloride salt. This differs from wheat-germ extraction and offers more consistent, scalable quality.
Yes, it is freely soluble in water, making it suitable for liquid or beverage-format products in addition to capsules, tablets, and powders. We can advise on basic formulation considerations such as pH and stability upon request.
Yes, small samples with a full CoA and safety data sheet are available for evaluation purposes. Please contact us with your intended application to arrange a sample shipment.
Yes, we fully support independent verification. Samples can be shipped directly to a testing laboratory of your choice, and we can provide the reference standard information needed for comparison.
Yes, we offer OEM/private-label support including custom formulation ratios, finished-dose forms (capsule, tablet, powder), and customer-branded packaging and labeling.
Spermidine trihydrochloride powder pack options are 1 kg, 5 kg, 25 kg. Special inner-packaging, labeling and transport requirements are agreed separately for the order.
The raw-material MOQ for spermidine trihydrochloride powder is 1 kg, with pack options of 1 kg, 5 kg, 25 kg. Quote the salt separately from free-base spermidine or plant extracts.
Define whether the target is salt mass or spermidine moiety, then use the supplied composition and suitable mass assay. Premix with a compatible carrier and verify unit content and filling-stage uniformity. Plant-extract weights and free-base doses are not directly interchangeable with trihydrochloride powder.
Documents are subject to product and batch availability; third-party reports are provided only when applicable.
Quality documents
The specification sheet defines acceptance limits. The COA shows actual batch results, which may vary by batch. Third-party reports are provided only for products or batches that have been independently tested.
Stable acceptance criteria · Available on request
Actual test data · Check the COA for the applicable batch
Technical Resources
A buyer-focused guide to sourcing spermidine trihydrochloride: confirm the salt form and assay basis, review the lot COA and handling data, compare samples to commercial lots, and plan moisture-controlled packaging.
If you've handled raw spermidine, you know the smell — and if you're building a capsule or tablet around it, that smell doesn't stay in the raw material bag. Here's the chemistry behind it and what actually works to manage it in a finished product.
Interested in Spermidine?
Our R&D team will respond to your inquiry or sample request within 24 hours.
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Alex Huang · Mon–Fri 9am–6pm CST
