ERGO
Ergothioneine
Key Specifications
| CAS Number | 497-30-3 |
| Purity | ≥98.0% |
| Molecular Formula | C₉H₁₅N₃O₂S |
| Molecular Weight | 229.30 g/mol |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| Storage | Room temperature, protect from light |
| Shelf Life | 24 months |
CAS 497-30-3
What Is ERGO?
Ergothioneine (EGT, CAS 497-30-3) is a naturally occurring sulfur-containing amino acid derivative of histidine (C9H15N3O2S, MW 229.30 g/mol), structurally classified as a 2-thioimidazole betaine. It was first identified in the ergot fungus Claviceps purpurea and occurs naturally in select fungi (including many culinary mushrooms such as oyster, shiitake, and king oyster mushroom) and in Actinobacteria (e.g., Mycobacterium species); plants and animals cannot synthesize it themselves and instead accumulate it from dietary sources via a dedicated transporter. Commercial bulk ergothioneine is increasingly produced by microbial fermentation using engineered production strains (such as recombinant E. coli, Corynebacterium glutamicum, or yeast) carrying the ergothioneine biosynthetic gene cluster, followed by cell lysis, purification, and crystallization to high purity, offering a more scalable and consistent alternative to mushroom extraction or multi-step chemical synthesis.
Other Names
Key Features
High-Purity Assay (≥98.0%)
Each batch is quantified by validated HPLC methods to confirm ergothioneine content meets or exceeds 98.0%, supporting consistent potency for dietary supplement and cosmetic formulations.
Fermentation-Based Production Control
Produced via engineered microbial fermentation under tightly controlled bioprocess parameters (strain, media, temperature, pH), giving more consistent batch-to-batch quality than mushroom extraction or classical multi-step chemical synthesis.
White Crystalline Powder Form
Supplied as a free-flowing white to off-white crystalline powder that is easy to weigh, blend, and dose accurately in capsules, tablets, powders, and cosmetic formulations.
Comprehensive Contaminant Testing
Every lot is screened for heavy metals (lead, arsenic, mercury, cadmium) and microbial limits (total plate count, yeast & mold, E. coli, Salmonella) to help ensure suitability for food, supplement, and cosmetic applications.
Flexible Bulk & OEM Supply
Available in bulk quantities with flexible packaging and OEM/private-label support, including customized specifications, documentation (COA, ISO), and packaging formats to match customer formulation and branding needs.
| Molecular Formula | C₉H₁₅N₃O₂S |
| Molecular Weight | 229.30 g/mol |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| Storage Condition | Room temperature, protect from light |
| Shelf Life | 24 months |
| Grade 1 | Supplement Grade · ≥98.0% · COA |
End-Product Applications
Mechanism & Benefits
Natural thiol antioxidant. Scavenges free radicals, protects cells from oxidative damage, delays aging.
Packaging Options
100 g 500 g 1 kg
Lead Time
7–10 business days
Minimum Order
100 g
Custom Packaging
Custom packaging (foil bags, HDPE drums), GMP labeling, and export documentation are available. Contact us for specifics.Certifications
ISO
ISO
Manufacturing Process
Production Strain Preparation
An engineered microbial strain (e.g., recombinant E. coli, Corynebacterium glutamicum, or yeast) carrying the ergothioneine biosynthetic gene cluster is recovered from seed stock and expanded through seed culture stages under aseptic conditions.
Fermentation
The seed culture is transferred into production bioreactors with optimized carbon, nitrogen, and sulfur sources; temperature, pH, dissolved oxygen, and feeding are controlled to drive intracellular biosynthesis and accumulation of ergothioneine.
Cell Harvest and Lysis
Fermentation broth is harvested and biomass is separated by centrifugation or filtration; cells are then disrupted (e.g., by high-pressure homogenization) to release intracellular ergothioneine into solution.
Clarification and Extraction
Cell debris and insoluble matter are removed by further centrifugation and/or membrane filtration, yielding a clarified extract enriched in ergothioneine.
Purification (Chromatography/Ion Exchange)
The clarified extract is purified using ion-exchange resins and/or chromatographic separation to remove residual proteins, salts, pigments, and process-related impurities.
Crystallization and Isolation
The purified solution is concentrated and crystallized under controlled conditions to yield ergothioneine crystals, which are then separated by centrifugation or filtration.
Drying, Milling and Quality Control
Crystals are dried, milled to the target particle size, and screened, with each batch tested against release specifications (purity, appearance, heavy metals, microbial limits) before packaging.
Quality Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to off-white crystalline powder | White crystalline powder |
| Odor & Color (1% solution) | Odorless; clear, colorless to pale yellow | Odorless; clear colorless |
| Identification (HPLC) | Conforms to reference standard | Conforms |
| Assay / Purity (HPLC) | ≥98.0% | 98.6% |
| Crystal Form / Particle Size | Fine crystalline powder, through 80 mesh | Through 80 mesh |
| Loss on Drying (Moisture) | ≤5.0% | 2.3% |
| Lead (Pb) | ≤2 mg/kg | <1 mg/kg |
| Arsenic (As) | ≤1 mg/kg | <0.5 mg/kg |
| Mercury (Hg) | ≤0.1 mg/kg | <0.05 mg/kg |
| Cadmium (Cd) | ≤1 mg/kg | <0.5 mg/kg |
| Total Plate Count | ≤1,000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative /g | Negative |
| Salmonella | Negative /25 g | Negative |
Purity & Assay Method
Ergothioneine purity is determined by reversed-phase HPLC with UV detection, quantified against a certified reference standard and reported on an as-is (or dried) basis. Buyers should request the certificate of analysis for the specific batch and confirm the HPLC method, reference standard, and acceptance criteria (≥98.0%) before finalizing an order, as well as whether results are reported on an anhydrous basis.
Solubility & Formulation
Ergothioneine is freely soluble in water, making it well suited for aqueous-based formulations such as beverages, oral liquids, effervescent powders, and water-phase cosmetic emulsions and serums. Because it carries no strong odor or color, it can typically be incorporated without masking agents, though final products should be evaluated for compatibility with other actives, pH range, and heat exposure during processing to preserve stability and potency.
Available Product Forms
Bulk ERGO Powder
High-purity raw powder for supplement factories, research organizations, ingredient distributors, and contract manufacturers.
ERGO Capsules
Custom capsule strength, shell material, bottle count, excipients, and label design for private-label brands.
ERGO Tablets
Tablet formulation, granulation, compression, coating, bottle packaging, and custom labeling support.
ERGO Powder Blends
ERGO can be blended with complementary actives (antioxidants, other longevity ingredients, vitamins, minerals) per customer formula.
What purity level does your Ergothioneine meet, and can you provide a Certificate of Analysis?+
Our Ergothioneine (CAS 497-30-3) is supplied at ≥98.0% purity as determined by HPLC. A batch-specific Certificate of Analysis (COA) covering assay, appearance, heavy metals, and microbial limits is provided with every shipment and can be shared in advance upon request.
How is your Ergothioneine produced — is it fermentation-derived or chemically synthesized?+
Our Ergothioneine is manufactured using a microbial fermentation and enzymatic production platform, in which an engineered production strain synthesizes ergothioneine, which is then extracted, purified, and crystallized. This bio-based route offers more consistent, scalable, and traceable production compared with mushroom extraction or classical chemical synthesis.
Is Ergothioneine soluble in water, and how should it be formulated?+
Yes, Ergothioneine is freely soluble in water and works well in aqueous formulations such as beverages, oral liquids, and water-phase cosmetic products. We recommend confirming compatibility with your specific formulation pH and other actives during your own stability testing.
Can we request a free sample before placing a bulk order?+
Yes, small samples are available on request so you can independently verify purity, appearance, and solubility before committing to a bulk purchase. Please contact our sales team with your intended application to arrange a sample.
Do you support independent third-party testing of your Ergothioneine?+
Yes, we welcome customers to send samples or retained batch samples to their own qualified third-party laboratories for independent verification of purity, heavy metals, and microbial limits alongside our internal COA.
Do you offer OEM or private-label services for Ergothioneine-based products?+
Yes, we support OEM and private-label programs, including customized packaging, labeling, and documentation, and can work with your team on specification requirements for finished dietary supplement or cosmetic products.
What packaging options are available for bulk Ergothioneine?+
Standard packaging is typically 1 kg or 5 kg per aluminum foil bag or drum with double-layer protection against moisture and light; other packaging sizes and formats can be arranged to match your logistics and storage needs.
What is the minimum order quantity (MOQ), and how can I get a quotation?+
MOQ varies depending on packaging format and intended use; please contact our sales team with your target quantity and application to receive a formal quotation and lead-time estimate.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Batch-specific COA & full traceability on every shipment
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party laboratory testing
Documents Available
- ·Certificate of Analysis (COA)
- ·Product specification sheet
- ·Material Safety Data Sheet (MSDS)
- ·Manufacturing flow chart
- ·HPLC test report
- ·Heavy-metal test report
- ·Microbiological test report
- ·Non-GMO / allergen statement
- ·Country-of-origin documentation
- ·Packing list & commercial invoice
Certificate availability should be confirmed with our sales team before ordering.
Sample Certificate of Analysis
PathGene Biotech Co., Ltd.
南京派琪生物科技有限公司
CERTIFICATE OF ANALYSIS
Layout example — not an issued batch record
Product Name
ERGO
CAS No.
497-30-3
Batch No.
PG-SAMPLE-000000
Packaging
Issued upon order confirmation
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White to off-white crystalline powder | White crystalline powder |
| Odor & Color (1% solution) | Odorless; clear, colorless to pale yellow | Odorless; clear colorless |
| Identification (HPLC) | Conforms to reference standard | Conforms |
| Assay / Purity (HPLC) | ≥98.0% | 98.6% |
| Crystal Form / Particle Size | Fine crystalline powder, through 80 mesh | Through 80 mesh |
| Loss on Drying (Moisture) | ≤5.0% | 2.3% |
| Lead (Pb) | ≤2 mg/kg | <1 mg/kg |
| Arsenic (As) | ≤1 mg/kg | <0.5 mg/kg |
| Mercury (Hg) | ≤0.1 mg/kg | <0.05 mg/kg |
| Cadmium (Cd) | ≤1 mg/kg | <0.5 mg/kg |
| Total Plate Count | ≤1,000 CFU/g | <100 CFU/g |
| Yeast & Mold | ≤100 CFU/g | <10 CFU/g |
| E. coli | Negative /g | Negative |
| Salmonella | Negative /25 g | Negative |
Conclusion: Conforms to internal quality specifications
The COA issued with your order will carry the actual batch number, manufacture/test dates, and QC signature.
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Interested in ERGO?
Our R&D team will respond to your inquiry or sample request within 24 hours.
