1. Chemical identity reference
Public chemical databases list Thionicotinamide Adenine Dinucleotide under the stated identity; this reference supports identity review and does not replace the batch COA.
Citation: PubChem compound recordView source
Sample Available
Thio-NAD
CAS 4090-29-3
Thionicotinamide Adenine Dinucleotide Powder
Purity≥95% β-Thio-NAD
Thionicotinamide adenine dinucleotide for enzyme assays and cofactor research
Thionicotinamide Adenine Dinucleotide Powder (CAS 4090-29-3), specification ≥95% β-Thio-NAD. Oxidized beta-Thio-NAD supplied for raw-material evaluation in enzyme assays and analytical-method development. Batch COA and evaluation samples are available. MOQ: 1 g. NAD+ ingredients
Quote / sample & deliveryMW679.49 g/mol
Key Specifications
| Product Positioning | Thionicotinamide adenine dinucleotide for enzyme assays and cofactor research |
| Application Scenarios | redox enzyme assays · cofactor research · diagnostic reagent development |
| CAS Number | 4090-29-3 |
| Purity | ≥95% β-Thio-NAD |
| Molecular Formula | C21H27N7O13SP2 |
| Molecular Weight | 679.49 g/mol |
| Appearance | Yellow powder |
| Solubility | Soluble in water; protect from light and confirm pH |
| Storage | Sealed, cool, dry and protected from light |
| Shelf Life | 2028-06-27 |
Procurement & delivery
Supply details confirmed by intended use, volume and target market
Purity ≥95% β-Thio-NADCAS 4090-29-3
MOQ1 g
Lead Time3–5 business days, subject to qualification
Packaging1 g / 100 g / 1 kg / 25 kg
Free sample available
View quality documents ↓What Is Thio-NAD?
Thionicotinamide adenine dinucleotide (Thio-NAD) is a sulfur-modified NAD analogue used in enzyme and redox assay development. The material should be handled as a research cofactor with lot-specific purity and stability controls. No. Thio-NAD is a sulfur-modified analogue, not a different name for NAD+. Validate enzyme compatibility and the detection method with the specified oxidized cofactor before adopting it. Product image: AI-generated ingredient illustration; actual batch appearance is subject to the sample and COA.
Other Names
Thio-NADβ-Thio-NADThionicotinamide-adenine dinucleotideCAS 4090-29-3
Key Features
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Thio-NAD Bulk Supply
PathGene supplies Thio-NAD, CAS 4090-29-3, with specification ≥95% β-Thio-NAD.
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β-Thio-NAD for Enzyme Assays
Oxidized β-Thio-NAD for enzyme-assay and analytical-method development.
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Batch Quality Documentation
Batch COA supports the quality evaluation of Thio-NAD and the agreed purchasing specification.
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Evaluation Samples
Request Thio-NAD samples for incoming-quality evaluation and project trials.
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Supply and Packaging
MOQ: 1 g. Packaging options: 1 g, 100 g, 1 kg, 25 kg.
Recent batch test data
Test items, specifications and results for the listed batch are shown here. View or request a COA in the quality documents section.
Batch No.
PGTNAD-260628
Test date
2026-06-29
Specification
Q/PG-INHOUSE
Pack size
1 g; 5 g; 100 g
Storage
Sealed, cool, dry and protected from light
Conclusion
Conforms
| Test Item | Specification | Result |
|---|---|---|
| Purity | ≥95% | 98.2% |
| β-Thio-NAD content | ≥95% | 95.8% |
| Sodium ion | ≤1% | 0.11% |
| Water | ≤5% | 4.50% |
| pH | 2.0–4.0 | 2.77 |
View an available COA in the quality documents section. Check the document for its product form and batch; request a signed COA for your purchasing or shipment batch separately.
Application Directions
Enzyme catalysis
Diagnostic reagent raw materials
Thio-NAD development essentials
Use thio-NAD for enzyme cycling and assay development, separately from nutritional NAD⁺ ingredients; evaluate signals under actual reaction conditions.
98.2%
Purity
95.8%
β-Thio-NAD content
4.50%
Water
PGTNAD-260628 · Results apply to this lot; interpret purity, assay and water on their respective bases.
- Composition candidates:
- Screen the relevant dehydrogenase, NAD(H), substrate and buffer, comparing cycling amplification with noncycling controls.
- Processing priorities:
- Compare enzyme, substrate and cofactor concentrations, addition order and reaction time, using reagent blanks and known standards.
- Testing priorities:
- Identity, beta-form assay, blank signal, calibration, linear range, recovery and intra-/inter-run precision.
Thio-NAD formulation development: composition, processing and validation
Composition candidates, processing sequences and tests support pilot design. Confirm candidate excipients and processes using this material in the actual finished formulation.
Working solutions: salt identity, concentration and stocks
- Design working solutions by molarity: m = c × V × M / f, with c in mol/L, V in L, the verified salt/hydrate M in g/mol, and f the mass assay as supplied. HPLC area purity is not automatically f; establish concentrations and linear range before scale-up.
- Screen the relevant dehydrogenase, NAD(H), substrate and buffer, comparing cycling amplification with noncycling controls.
- Record ingredient lots, actual component masses, loading bases and addition sequence, retaining blanks and single-ingredient controls for traceable changes.
Reaction development: addition order, blanks and linearity
- Compare enzyme, substrate and cofactor concentrations, addition order and reaction time, using reagent blanks and known standards.
- Screen enzyme, substrate, cofactor and reaction time to establish the linear signal range; assess matrix effects before and after addition.
Validation and scale-up: tests, retained samples and packaging
- Identity, beta-form assay, blank signal, calibration, linear range, recovery and intra-/inter-run precision.
- Sample upper/middle/lower positions and start/middle/end filling; compare with ingredients, process blanks and single-ingredient results, documenting deviations and corrections.
- Include actual packaging, transport and intended storage in retained-sample plans; ingredient COAs and storage conditions do not establish a new finished-product shelf life.
Published reaction systems and research directions
Study combinations, individual-ingredient mechanisms and product precedents are distinguished; expand each entry for scope and pilot-development checks.
Enzyme-cycling assay studyThio-NAD + dehydrogenase cycling assay system
A practical research pairing is a thio-NAD cycling assay using 3α-hydroxysteroid dehydrogenase, its steroid substrate, and an NADH-coupled reverse step. Optimize enzyme activity, substrate blank, cofactor purity, pH, temperature and absorbance near 400 nm; use it as an analytical-method development lead rather than a nutritional blend.
Expand research and formulation notes +Collapse research and formulation notes −
Development rationale: A practical research pairing is a thio-NAD cycling assay using 3α-hydroxysteroid dehydrogenase, its steroid substrate, and an NADH-coupled reverse step. Optimize enzyme activity, substrate blank, cofactor purity, pH, temperature and absorbance near 400 nm; use it as an analytical-method development lead rather than a nutritional blend.
Evidence scope: A published ultrasensitive ELISA method used thio-NAD cycling with 3α-HSD and NADH to generate measurable thio-NADH. This supports an assay-system application, not efficacy or suitability in an oral product; validate with the intended enzyme, matrix and instrument.
Pilot checks: Include no-enzyme, no-substrate and reagent blanks; compare cofactor/enzyme levels and establish linearity, recovery and inter-lot precision.
2014 · Ultrasensitive enzyme-linked immunosorbent assay (ELISA) of proteins by combination with the thio-NAD cycling method. ↗Literature-Supported Research Directions
Scientific Research
2. Thio-NAD cycling in ultrasensitive immunoassays
A methods paper coupled ELISA with a thio-NAD/NADH enzyme-cycling reaction. Accumulated thio-NADH was monitored near 400 nm, demonstrating a research use of thio-NAD as an assay cofactor. Performance depends on the complete assay design and reagents, not on the cofactor alone.
Citation: Watabe et al. Ultrasensitive enzyme-linked immunosorbent assay (ELISA) of proteins by combination with the thio-NAD cycling method. Biophysics (Nagoya-shi, Japan) (2014). PMID: 27493498.View source
3. Nucleic-acid detection without amplification
A 2022 methods study combined probe hybridization with thio-NAD cycling for nucleic-acid detection without amplification. The reported work is an assay-development example; it does not certify this product as a diagnostic reagent or establish a clinical diagnostic performance claim.
Citation: Yamura et al. Non-amplification nucleic acid detection with thio-NAD cycling. Journal of microbiological methods (2023). PMID: 36496031.View source
Note: For ingredient R&D and scientific information; findings depend on model, dose, formulation and population and do not constitute a treatment claim or efficacy guarantee.
Lead Time
3–5business days
Standard products are generally in stock; confirm the exact lead time with us.
Minimum Order
1 g
Samples, pilot trials, and commercial volumes are supported.
Foil Pouch
Sealed, moisture-protective inner packaging
Fiber Drum
Standard outer packaging for bulk transport
Bulk Shipping
Palletized and stretch-wrapped for protection
Ambient storage: foil pouch + fiber drum; temperature controlled: foil pouch + insulated box/carton. Confirm by product.
Custom packaging: Custom foil bags or HDPE drums, GMP label printing, and export documentation are available. Contact us for a quote.Images are packaging illustrations.
Certifications
Purity & Assay Method
The stated purity and batch result are shown separately. Confirm the method, dry-basis calculation and salt or hydrate form against the current COA.
Solubility & Formulation
Formulation work should confirm concentration, pH, light, moisture and hold-time controls for Thionicotinamide Adenine Dinucleotide; suitability depends on the final format and market requirements.
What are the MOQ and pack options for Thio-NAD powder?+
The raw-material MOQ for Thio-NAD powder is 1 g, with pack options of 1 g, 100 g, 1 kg, 25 kg. Method-development orders should specify oxidized beta-Thio-NAD separately from NAD+.
Can Thio-NAD replace NAD+ in an assay without validation?+
No. Thio-NAD is a sulfur-modified analogue, not a different name for NAD+. Validate enzyme compatibility and the detection method with the specified oxidized cofactor before adopting it.
What is the specification and development use of Thio-NAD powder?+
Oxidized beta-Thio-NAD powder, CAS 4090-29-3, is specified at ≥95% beta-Thio-NAD and supplied for enzyme-assay and analytical-method development. Match oxidation state, enzyme compatibility and detection conditions to the project, with review of the batch COA.
Which performance characteristics should be validated for a Thio-NAD assay?+
Validate reaction rate, detection response, linear range, blanks and stability with the specified enzyme, substrate and buffer. Thio-NAD is a sulfur-modified NAD+ analogue. Reassess these characteristics when changing cofactors rather than transferring NAD+ assay conclusions directly.
Why Choose PathGene
- Direct China manufacturer supply — factory pricing, no middleman
- Recent-batch COA & batch traceability
- In-house R&D + synthetic biology / enzymatic production platform
- Free sample for qualified B2B buyers before commercial order
- Flexible MOQ — from lab-scale trial to ton-scale commercial supply
- One-stop OEM/private-label support for finished dosage forms
- Worldwide shipping by courier, air freight, and sea freight
OEM & Private-Label Services
- ·Custom capsule manufacturing
- ·Custom tablet manufacturing
- ·Powder blending & custom formulation
- ·Private-label bottles, pouches & labels
- ·Small trial orders
- ·Bulk commercial production
- ·Export documentation
- ·Third-party testing support available on request
Documents Available
- ·Product specification sheet — request from sales
- ·Recent-batch COA — confirm by product and batch
- ·Other quality documents — confirm by product and batch
Documents are subject to product and batch availability; third-party reports are provided only when applicable.
Quality documents
Review specifications first, then batch data at shipment
The specification sheet defines acceptance limits. The COA shows actual batch results, which may vary by batch. Third-party reports are provided only for products or batches that have been independently tested.
Product specification
Stable acceptance criteria · Available on request
Recent-batch COA
Actual test data · Check the COA for the applicable batch
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