P5P (PLP)
CAS 41468-25-1
Purity≥98.0%
Active vitamin B6 coenzyme for enzyme and nutrition formulations
For B2B ingredient sourcing, request the specification, batch COA, sample, packaging and lead-time confirmation. Explore nutrition & functional ingredients.
Confirm monohydrate form and PLP assay basis.
Key Specifications
| Product Positioning | Active vitamin B6 coenzyme for enzyme and nutrition formulations |
| Application Scenarios | PLP-dependent enzyme assays · active-B6 premixes · nutrition tablet development |
| CAS Number | 41468-25-1 |
| Purity | ≥98.0% |
| Molecular Formula | C₈H₁₂NO₇P |
| Molecular Weight | 265.16 g/mol |
| Appearance | Light yellow to yellow crystalline powder |
| Solubility | Soluble in water |
| Storage | 2–8°C, protect from light |
| Shelf Life | 24 months |
Procurement & delivery
Supply details confirmed by intended use, volume and target market
Pyridoxal 5'-phosphate monohydrate (P5P/PLP, CAS 41468-25-1) is the monohydrate form of the biologically active, phosphorylated coenzyme form of vitamin B6. Its molecular formula is C8H12NO7P and molecular weight is 265.16 g/mol. In the body, PLP is generated from vitamin B6 precursors (pyridoxine, pyridoxal, and pyridoxamine) through kinase- and oxidase-mediated metabolism. For commercial supply, buyers should confirm the exact hydrate form, identity method, assay basis, and batch COA rather than treating anhydrous PLP and the monohydrate as interchangeable line items.
Pharmaceutical-Grade Purity
Assayed at ≥98.0% by HPLC and manufactured to meet USP monograph expectations, ensuring consistent potency batch to batch for pharmaceutical and nutraceutical formulators.
Controlled Biocatalytic Production
Produced from pyridoxine through a tightly controlled phosphorylation-oxidation process, leveraging our enzymatic and synthetic-biology production platform to improve reaction selectivity and reduce process impurities.
Consistent Crystalline Appearance
Supplied as a uniform light yellow to yellow crystalline powder with controlled particle size, supporting reliable weighing, blending, and downstream processing.
Rigorous Contaminant Screening
Every batch is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella), with full documentation available on request.
Flexible Bulk & OEM Supply
Available in flexible bulk quantities with customizable packaging, labeling, and OEM/private-label options to support supplement, pharmaceutical, and premix manufacturers of varying scale.
Test items, specifications and batch results are shown here; request the formal COA through the card below.
Batch No.
PGPLP-260613
Test date
2026-06-15
Specification
In-house
Pack size
25kg/drum
Storage
2–8°C, protect from light
Conclusion
Conforms
| Test Item | Specification | Result |
|---|---|---|
| Appearance | Light yellow to yellow crystalline powder | Conforms, light yellow crystalline powder |
| Odor | Odorless | Odorless |
| Identification (IR) | Conforms to reference spectrum | Conforms |
| Assay (HPLC) | ≥98.0% | 98.6% |
| pH (1% aqueous solution) | 4.5-6.0 | 5.2 |
| Loss on drying | ≤1.0% | 0.4% |
| Residue on ignition | ≤0.5% | 0.2% |
| Heavy metals (Pb) | ≤10 ppm | <2 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Total plate count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & mold | ≤100 CFU/g | <10 CFU/g |
| E. coli / Salmonella | Negative/25g | Negative |
This page shows a batch-data summary only. No direct COA file link is provided; request the signed document through the form below.
Hard capsules
Powders
Softgels
Tablets
Sublingual tablets
Oral dissolving films
Literature-Supported Research Directions
Pyridoxal-5′-phosphate (PLP) is the active vitamin-B6 coenzyme for transaminases, decarboxylases and amino-acid metabolism. It supports research into neurotransmitter synthesis, homocysteine metabolism and fortified-nutrition formulations.
Citation: Ueland et al., Clinical Chemistry and Laboratory Medicine (2017), review of vitamin B6 vitamers and PLP biomarkers.View source
Note: For ingredient R&D and scientific information; findings depend on model, dose, formulation and population and do not constitute a treatment claim or efficacy guarantee.
Lead Time
3–5business days
Standard products are generally in stock; confirm the exact lead time with us.
Minimum Order
1 kg
Samples, pilot trials, and commercial volumes are supported.
Foil Pouch
Sealed, moisture-protective inner packaging
Insulated Box + Carton
For products requiring temperature-controlled transport
Bulk Shipping
Palletized and stretch-wrapped for protection
Ambient storage: foil pouch + fiber drum; temperature controlled: foil pouch + insulated box/carton. Confirm by product.
Custom packaging: Custom foil bags or HDPE drums, GMP label printing, and export documentation are available. Contact us for a quote.Images are packaging illustrations.
Certifications
USP
US Pharmacopoeia
Precursor Sourcing
High-purity pyridoxine (vitamin B6) is sourced and qualified as the starting precursor, with incoming material verified against internal specifications before entering the production line.
Biocatalyst/Reagent Preparation
Pyridoxal kinase and pyridoxine-5'-phosphate oxidase biocatalysts (or, in the conventional route, chemical phosphorylating reagents) are prepared and activity-verified prior to use.
Phosphorylation
Pyridoxine is enzymatically (or chemically) phosphorylated at the 5'-hydroxymethyl position to generate pyridoxine-5'-phosphate, with ATP or an equivalent phosphoryl donor supplied and regenerated in situ for the biocatalytic route.
Oxidative Conversion
Pyridoxine-5'-phosphate is oxidized at the C4 position — via pyridoxine-5'-phosphate oxidase in the biocatalytic route, or via a controlled chemical oxidant in the conventional route — to yield pyridoxal 5'-phosphate.
Reaction Monitoring & Endpoint Control
Conversion is monitored in real time (e.g., by HPLC/UV) to confirm reaction completion and minimize residual pyridoxine-5'-phosphate or over-oxidation byproducts.
Clarification & Biocatalyst Removal
The reaction mixture is clarified by filtration/centrifugation to remove spent enzyme, cell debris, or insoluble reaction residues from the process stream.
Purification & Crystallization
The clarified solution is purified (e.g., ion-exchange or chromatographic polishing) and subjected to controlled crystallization to obtain PLP crystals of consistent form and particle size.
Drying, Milling & Quality Release
Crystals are dried under controlled temperature and light-protected conditions, milled to target particle size, then sampled for full QC testing before batch release and packaging.
Purity is determined by high-performance liquid chromatography (HPLC) with UV detection, typically referenced against a USP or in-house PLP working standard at approximately 325 nm, with assay reported on an as-is basis. Buyers should request the current certificate of analysis (COA) for each batch and confirm the reference standard, column/method, and acceptance criteria used, particularly if the material is intended for pharmacopeial-grade formulations.
PLP is soluble in water, which supports its use in aqueous-based dietary supplement formulations (liquids, effervescent tablets, oral solutions) as well as clinical nutrition applications where water solubility is required; it is generally poorly soluble in most organic solvents. Because PLP is light- and moisture-sensitive and may undergo gradual degradation in aqueous solution — particularly at extremes of pH or under prolonged UV/visible light exposure — formulators should consider light-protected packaging, controlled pH buffering, and appropriate storage conditions, and are encouraged to run compatibility and stability studies specific to their finished-product matrix.
We provide a current certificate of analysis (COA) for every batch, including HPLC assay, appearance, heavy metals, and microbial limits. We recommend reviewing the COA against your internal specification and, for larger orders, requesting a pre-shipment sample for independent verification.
Our PLP is manufactured starting from pyridoxine (vitamin B6) through a controlled phosphorylation and oxidation sequence, leveraging our enzymatic/biocatalytic production platform alongside conventional chemical processing steps, followed by purification and crystallization.
Yes, PLP is soluble in water. It is commonly formulated into aqueous-based supplements, tablets, and clinical nutrition products; given its light- and moisture-sensitivity, we recommend light-protected packaging and pH-appropriate buffering, and encourage formulation-specific stability testing.
Yes, small samples are available for qualification and testing purposes upon request; please contact our sales team with your intended application and required quantity.
In addition to our internal COA, we support submission of samples to accredited third-party laboratories chosen by the customer, and can help coordinate additional testing (e.g., heavy metals, microbial, residual solvents) as required for your market's regulatory needs.
Yes, we support OEM and private-label supply, including custom packaging, labeling, and documentation, for supplement brands, pharmaceutical formulators, and premix manufacturers.
Standard packaging is in light-protected, moisture-resistant containers (e.g., aluminum foil bags or drums with inner liners) in various sizes; custom packaging sizes and materials can be arranged for larger or recurring orders.
MOQ depends on packaging format and intended application; please contact our sales team with your target volume, purity requirement, and destination to receive a tailored quotation.
Documents are subject to product and batch availability; third-party reports are provided only when applicable.
Quality documents
The specification sheet defines acceptance limits. The COA shows actual batch results, which may vary by batch. Third-party reports are provided only for products or batches that have been independently tested.
Stable acceptance criteria · Available on request
Actual test data · Issued with each shipment
Technical Resources
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Alex Huang · Mon–Fri 9am–6pm CST
