P5P (PLP)
CAS 41468-25-1
Purity≥98.0%
Active vitamin B6 coenzyme for enzyme and nutrition formulations
Pyridoxal 5'-Phosphate Monohydrate Powder (CAS 41468-25-1), specification ≥98.0%. Pyridoxal phosphate monohydrate (P5P or PLP), a vitamin B6-related ingredient distinct from pyridoxine hydrochloride. Batch COA and evaluation samples are available. MOQ: 1 kg. nutrition & functional ingredients
Quote / sample & delivery
Key Specifications
| Product Positioning | Active vitamin B6 coenzyme for enzyme and nutrition formulations |
| Application Scenarios | PLP-dependent enzyme assays · active-B6 premixes · nutrition tablet development |
| CAS Number | 41468-25-1 |
| Purity | ≥98.0% |
| Molecular Formula | C₈H₁₂NO₇P |
| Molecular Weight | 265.16 g/mol |
| Appearance | Light yellow to yellow crystalline powder |
| Solubility | Soluble in water |
| Storage | 2–8°C, protect from light |
| Shelf Life | 24 months |
Procurement & delivery
Supply details confirmed by intended use, volume and target market
Pyridoxal 5'-phosphate monohydrate (P5P/PLP, CAS 41468-25-1) is the monohydrate form of the biologically active, phosphorylated coenzyme form of vitamin B6. Its molecular formula is C8H12NO7P and molecular weight is 265.16 g/mol. In the body, PLP is generated from vitamin B6 precursors (pyridoxine, pyridoxal, and pyridoxamine) through kinase- and oxidase-mediated metabolism. For commercial supply, buyers should confirm the exact hydrate form, identity method, assay basis, and batch COA rather than treating anhydrous PLP and the monohydrate as interchangeable line items.
Pharmaceutical-Grade Purity
Assayed at ≥98.0% by HPLC and manufactured to meet USP monograph expectations, ensuring consistent potency batch to batch for pharmaceutical and nutraceutical formulators.
Controlled Biocatalytic Production
Produced from pyridoxine through a tightly controlled phosphorylation-oxidation process, leveraging our enzymatic and synthetic-biology production platform to improve reaction selectivity and reduce process impurities.
Consistent Crystalline Appearance
Supplied as a uniform light yellow to yellow crystalline powder with controlled particle size, supporting reliable weighing, blending, and downstream processing.
Rigorous Contaminant Screening
Every batch is screened for heavy metals (Pb, Hg, Cd, As) and microbial limits (total plate count, yeast/mold, E. coli, Salmonella), with full documentation available on request.
Flexible Bulk & OEM Supply
Available in flexible bulk quantities with customizable packaging, labeling, and OEM/private-label options to support supplement, pharmaceutical, and premix manufacturers of varying scale.
Test items, specifications and results for the listed batch are shown here. View or request a COA in the quality documents section.
Batch No.
PGPLP-260613
Test date
2026-06-15
Specification
In-house
Pack size
25kg/drum
Storage
2–8°C, protect from light
Conclusion
Conforms
| Test Item | Specification | Result |
|---|---|---|
| Appearance | Light yellow to yellow crystalline powder | Conforms, light yellow crystalline powder |
| Odor | Odorless | Odorless |
| Identification (IR) | Conforms to reference spectrum | Conforms |
| Assay (HPLC) | ≥98.0% | 98.6% |
| pH (1% aqueous solution) | 4.5-6.0 | 5.2 |
| Loss on drying | ≤1.0% | 0.4% |
| Residue on ignition | ≤0.5% | 0.2% |
| Heavy metals (Pb) | ≤10 ppm | <2 ppm |
| Arsenic (As) | ≤2 ppm | <1 ppm |
| Mercury (Hg) | ≤1 ppm | <0.5 ppm |
| Cadmium (Cd) | ≤1 ppm | <0.5 ppm |
| Total plate count | ≤1000 CFU/g | <100 CFU/g |
| Yeast & mold | ≤100 CFU/g | <10 CFU/g |
| E. coli / Salmonella | Negative/25g | Negative |
View an available COA in the quality documents section. Check the document for its product form and batch; request a signed COA for your purchasing or shipment batch separately.
Hard capsules
Powders
Softgels
Tablets
Sublingual tablets
Oral dissolving films
P5P monohydrate is distinct from pyridoxine; calculate vitamin B6 equivalents separately from hydrate loading.
98.6%
Assay (HPLC)
PGPLP-260613 · Results apply to this lot; interpret purity, assay and water on their respective bases.
Composition candidates, processing sequences and tests support pilot design. Confirm candidate excipients and processes using this material in the actual finished formulation.
Study combinations, individual-ingredient mechanisms and product precedents are distinguished; expand each entry for scope and pilot-development checks.
Pyridoxal-5-phosphate and magnesium have been combined in nutritional research formulations. Development should specify the magnesium salt, elemental magnesium and total vitamin B6 exposure.
Expand research and formulation notes +Collapse research and formulation notes −Development rationale: Pyridoxal-5-phosphate and magnesium have been combined in nutritional research formulations. Development should specify the magnesium salt, elemental magnesium and total vitamin B6 exposure.
Evidence scope: The cited randomized trial evaluated magnesium-pyridoxal-5-phosphate-glutamate, a particular compound. It does not establish equivalent effects for an arbitrary magnesium salt blended with P5P. Define the actual material, elemental magnesium, vitamin B6 declaration and compatibility before using this as a formulation benchmark.
Pilot checks: Include no-enzyme, no-substrate and reagent blanks; compare cofactor/enzyme levels and establish linearity, recovery and inter-lot precision.
View original research or document ↗Literature-Supported Research Directions
Pyridoxal-5′-phosphate (PLP) is the active vitamin-B6 coenzyme for transaminases, decarboxylases and amino-acid metabolism. It supports research into neurotransmitter synthesis, homocysteine metabolism and fortified-nutrition formulations.
Citation: Ueland et al. Direct and Functional Biomarkers of Vitamin B6 Status. Annual review of nutrition (2015). PMID: 25974692.View source
Note: For ingredient R&D and scientific information; findings depend on model, dose, formulation and population and do not constitute a treatment claim or efficacy guarantee.
Lead Time
3–5business days
Standard products are generally in stock; confirm the exact lead time with us.
Minimum Order
1 kg
Samples, pilot trials, and commercial volumes are supported.
Foil Pouch
Sealed, moisture-protective inner packaging
Insulated Box + Carton
For products requiring temperature-controlled transport
Bulk Shipping
Palletized and stretch-wrapped for protection
Ambient storage: foil pouch + fiber drum; temperature controlled: foil pouch + insulated box/carton. Confirm by product.
Custom packaging: Custom foil bags or HDPE drums, GMP label printing, and export documentation are available. Contact us for a quote.Images are packaging illustrations.
Certifications
USP
US Pharmacopoeia
Precursor Sourcing
High-purity pyridoxine (vitamin B6) is sourced and qualified as the starting precursor, with incoming material verified against internal specifications before entering the production line.
Biocatalyst/Reagent Preparation
Pyridoxal kinase and pyridoxine-5'-phosphate oxidase biocatalysts (or, in the conventional route, chemical phosphorylating reagents) are prepared and activity-verified prior to use.
Phosphorylation
Pyridoxine is enzymatically (or chemically) phosphorylated at the 5'-hydroxymethyl position to generate pyridoxine-5'-phosphate, with ATP or an equivalent phosphoryl donor supplied and regenerated in situ for the biocatalytic route.
Oxidative Conversion
Pyridoxine-5'-phosphate is oxidized at the C4 position — via pyridoxine-5'-phosphate oxidase in the biocatalytic route, or via a controlled chemical oxidant in the conventional route — to yield pyridoxal 5'-phosphate.
Reaction Monitoring & Endpoint Control
Conversion is monitored in real time (e.g., by HPLC/UV) to confirm reaction completion and minimize residual pyridoxine-5'-phosphate or over-oxidation byproducts.
Clarification & Biocatalyst Removal
The reaction mixture is clarified by filtration/centrifugation to remove spent enzyme, cell debris, or insoluble reaction residues from the process stream.
Purification & Crystallization
The clarified solution is purified (e.g., ion-exchange or chromatographic polishing) and subjected to controlled crystallization to obtain PLP crystals of consistent form and particle size.
Drying, Milling & Quality Release
Crystals are dried under controlled temperature and light-protected conditions, milled to target particle size, then sampled for full QC testing before batch release and packaging.
Purity is determined by high-performance liquid chromatography (HPLC) with UV detection, typically referenced against a USP or in-house PLP working standard at approximately 325 nm, with assay reported on an as-is basis. Buyers should request the current certificate of analysis (COA) for each batch and confirm the reference standard, column/method, and acceptance criteria used, particularly if the material is intended for pharmacopeial-grade formulations.
PLP is soluble in water, which supports its use in aqueous-based dietary supplement formulations (liquids, effervescent tablets, oral solutions) as well as clinical nutrition applications where water solubility is required; it is generally poorly soluble in most organic solvents. Because PLP is light- and moisture-sensitive and may undergo gradual degradation in aqueous solution — particularly at extremes of pH or under prolonged UV/visible light exposure — formulators should consider light-protected packaging, controlled pH buffering, and appropriate storage conditions, and are encouraged to run compatibility and stability studies specific to their finished-product matrix.
We provide a current certificate of analysis (COA) for every batch, including HPLC assay, appearance, heavy metals, and microbial limits. We recommend reviewing the COA against your internal specification and, for larger orders, requesting a pre-shipment sample for independent verification.
Our PLP is manufactured starting from pyridoxine (vitamin B6) through a controlled phosphorylation and oxidation sequence, leveraging our enzymatic/biocatalytic production platform alongside conventional chemical processing steps, followed by purification and crystallization.
Pyridoxal 5-phosphate monohydrate (P5P/PLP) is slightly soluble in water and soluble in alkaline solution, as described in COA PGPLP-260613. Test the intended concentration, solvent, pH and temperature, and separately assess processing and storage stability.
Yes, small samples are available for qualification and testing purposes upon request; please contact our sales team with your intended application and required quantity.
In addition to our internal COA, we support submission of samples to accredited third-party laboratories chosen by the customer, and can help coordinate additional testing (e.g., heavy metals, microbial, residual solvents) as required for your market's regulatory needs.
Yes, we support OEM and private-label supply, including custom packaging, labeling, and documentation, for supplement brands, pharmaceutical formulators, and premix manufacturers.
P5P monohydrate powder pack options are 1 kg, 5 kg, 25 kg. Special inner-packaging, labeling and transport requirements are agreed separately for the order.
The raw-material MOQ for P5P monohydrate powder is 1 kg, with pack options of 1 kg, 5 kg, 25 kg. Typical lead time is 3–5 business days, with dispatch confirmed for the order.
Both commonly refer to pyridoxal 5'-phosphate. For the supplied powder, also specify monohydrate and CAS 41468-25-1; an abbreviation alone does not define hydration.
CAS 41468-25-1 identifies the monohydrate; CAS 54-47-7 identifies the parent pyridoxal 5'-phosphate entry. They differ in formula weight and hydration, so specifications and assay calculations must identify the supplied form.
Keep the reported value and its specification. Batch PGPLP-260613 lists assay 100.4% on a dry basis, within 98.0-101.0%, with water 7.7%. Review the method and reference-standard assignment; do not present the result as an as-is mass fraction.
Documents are subject to product and batch availability; third-party reports are provided only when applicable.
Quality documents
The specification sheet defines acceptance limits. The COA shows actual batch results, which may vary by batch. Third-party reports are provided only for products or batches that have been independently tested.
Stable acceptance criteria · Available on request
Actual test data · Check the COA for the applicable batch
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